MOLECULAR IDENTIFICATION OF Salmonella Enterica FROM PATIENTS WITH DIARRHEA IN DUHOK GOVERNORATE KURDISTAN REGION / IRAQ
Keywords:
Salmonella enterica;, Food borne infections and RT-PCR;
Abstract
Background and objectives: Salmonella enterica are responsible for causing the highest number of bacterial foodborne infections in the world. Methods: The Present study was carried out for detection and molecular identification of Salmonella enterica isolated from stool specimens of patients suffering from diarrhoea using traditional method and identification by using of RT-Polymerase Chain Reaction (PCR) techniques. Results: Out of the 121 patients with diarrhoea attended Azadi and Heevi Teaching Hospitals, 72 cases (59.504%) gave positive results for culturing. While by PCR method, 68 cases (56.198%) gave positive results for Salmonella enterica. In this study, (49) cases (40.495%) of non-Salmonella spp. gave negative results by culture and (53) cases (43.8%) of non-Salmonella spp. gave negative results by PCR method. Conclusions: The current study showed that RT-PCR technique is more accurate and sensitive compared to traditional methods.Downloads
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2. Bonkoungou IJ, Haukka K, Österblad M, et al., 2013. Bacterial and viral etiology of childhood diarrhoea in Ouagadougou, Burkina Faso. BMC Pediatr.;13:36.
3. Cohen, H.J., Mechanda, S.M. and Lin, W., 1996. PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp. Appl. Environ. Microbiol., 62(12), pp.4303-4308.
4. Daum LT, Barnes WJ, McAvin JC, Neidert MS, Cooper LA, Huff WB, et al., 2002. Real-Time PCR detection of Salmonella in suspect foods from a gastroenteritis outbreak in Kerr County, Texas. J Clin Microbiol;40(8):3050–2.
5. Evans JA, Adusei A, Timmann C, May J, Mack D, Agbenyega T, Horstmann RD, Frimpong E., 2004. High mortality of infant bacteraemia clinically indistinguishable from severe malaria. Q J Med.; 97:591–7.
6. Ferre, M.R., Jakab, P.D. and Tieman, J.S., Visualization Tech Inc, 2002. Position tracking and imaging system for use in medical applications. U.S. Patent 6,445,943.
7. Havelaar AH, Kirk MD, Torgerson PR, et al., 2015. World Health Organization Global estimates and regional comparisons of the burden of foodborne disease in 2010. PLoS Med;12: e1001923.
8. International Standardization Organization: ISO 6579-1:2017.
9. Lynch, M.F., Blanton, E.M., Bulens, S., Polyak, C., Vojdani, J., Stevenson, J., Medalla, F., Barzilay, E., Joyce, K., Barrett, T. and Mintz, E.D., 2009. Typhoid fever in the United States, 1999-2006. Jama, 302(8), pp.859-865.
10. Malorny, B., J. Hoorfar, C. Bunge and R. Helmuth, 2003. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Applied Environ. Microbiol., 69: 290-296.
11. Oliveiria SD, Rodenbusch CR, Ce´ MC, Rocha SL, Canal CW., 2003. Evaluation of selective and non-selective enrichment PCR procedures for Salmonella detection. Lett Appl Microbiol ;36(4):217–21.
12. Owaied, d.y.h., 2016. The effect of enviromental conditions on the spreed of salmonella germs in human in the tikrit. Medical journal of tikrit, 21(1), pp.249-261.
13. Ranjbar R, Karami A, Farshad S, Giammanco GM, Mammina C., 2014. Typing methods used in the molecular epidemiology of microbial pathogens: a how-to guide. New Microbiol;37:1-15. PubMed PMID: 24531166.
14. Ranjbar R, Naghoni A, Farshad S, Lashini H, Najafi A, Sadeghifard N, et al., 2014. Use of TaqMan(R) real-time PCR for rapid detection of Salmonella enterica serovar Typhi. Acta Microbiol Immunol Hung; 61:121-30.doi: 10.1556/AMicr.61.2014.2.3. PubMed PMID: 24939681.
15. Riyaz-Ul-Hassan US, Verma V, Qazi GN., 2004. Rapid detection of salmonella by polymerase chain reaction. Mol Cell Probes;18(333):39.
16. Saba CKS, Escudero JA, Herrera-Leon S, Porrero MC, Suarez M, Dominguez L, Demuyakor B, Gonzalez-Zorn B., 2013. First identification of Salmonella Urbana and Salmonella Ouakam in humans in Africa. J Infect Dev Ctries.;7:691–5.
17. Wilkens J, Newman MJ, Commey JO, Seifert H., 1997. Salmonella bloodstream infection on Ghanaian children. Clin Microbiol Infect.;3:616–20.
Published
2019-11-04
How to Cite
ABDULKHALEQ SALIH, D., M. ABDO, D. J., & T. SAADI, D. A. (2019). MOLECULAR IDENTIFICATION OF Salmonella Enterica FROM PATIENTS WITH DIARRHEA IN DUHOK GOVERNORATE KURDISTAN REGION / IRAQ. Journal of Duhok University, 22(1), 148-154. https://doi.org/10.26682/sjuod.2019.22.1.16
Section
Pure and Engineering Sciences
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