AUGMENTATION OF SURGICALLY CREATED BONY DEFECTS USING BIPHASIC CALCIUM PHOSPHATE WITH AND WITHOUT PLATELET RICH FIBRIN: AN EXPERIMENTAL STUDY IN SHEEP

ALKAFARANI  W. M.; BABAN  L. F.

doi:10.26682/sjuod.2019.22.2.26

ALKAFARANI W. M. Dept.of oral and maxillofacialSurgeryand Periodontics, College of dentistry, University of Duhok, Kurdistan Region-Iraq
BABAN L. F. Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Hawler Medical University, Kurdistan Region-Iraq

DOI: https://doi.org/10.26682/sjuod.2019.22.2.26

Keywords: KEY WORDS: Platelet Rich Fibrin, Biphasic Calcium Phosphate, Bone Augmentation, Bone Healing. Abbreviation: (BCP) biphasic calcium phosphate. (PRF) platelet rich fibrin.

Abstract

ABSTRACT

Back ground and objective: The reconstruction of large bony defects is one of great challenge in clinical research, various materials and techniques were used in bone augmentation. Recently, autologous platelet rich fibrin (PRF) which contain various growth factors accelerates tissue healing and promotes bone regeneration. Therefore, this study aimed to evaluate the effectiveness of adding PRF to biphasic calcium phosphate (BCP) on healing process of iliac bone defects in sheep.

Materials and methods: six iliac bone defect 8mm in diameters and depth were created in each side of four sheep. The superior two defects were filled with blood as control group, the middle two defects were filled with BCP and the inferior two defects were filled with a mixture of BCP and PRF equally. The PRF was prepared by centrifuging sheep own blood at 2700rpm for 12minutes. One sheep was sacrificed at 2, 4, 6 and 12 postoperatively weeks, and twelve iliac bone block from each period were prepared histologically and the slides stained with masson trichrome, hematoxylin and eosin stains for examination of new bone formation, bone maturation and intensity of osteoblast and osteoclast over healing time periods.

Results: The study showed that the new bone formation percentage and the intensity of osteoblast and osteoclast cells were significantly increased in each group with healing time (p = <0.05) and they were in BCP+PRF group was higher than in BCP group and much higher than in control group at all-time intervals. Besides, the woven and lamellar bone percentages over time periods were significantly decreased and increased, respectively. Importantly, the study revealed that the (BCP+PRF) group had statistically significant increase in the percentage of new bone formation and lamellar bone and compared to the BCP and control groups in all time intervals.

Conclusion: Within the limitations of this experimental study, our results demonstrated that the addition of PRF to BCP increases the formation of new bone.

Downloads

Download data is not yet available.

References

REFERENCES
Abdelmagid, S.E., Shaaban, A.M.M., Ragaa, H., Nagui, D., 2015. Comparison between the Use of Platelet Rich Fibrin with/and Without Biphasic Calcium Phosphate for Osseointegration around Implants (Experimental Study). International Journal of Science and Research (IJSR); 6 (2): E1803-1807.
Abdullah, W.A., 2016. Evaluation of bone regenerative capacity in rats claverial bone defect using platelet rich fibrin with and without beta tri calcium phosphate bone graft material. Saudi Dent J; 28 (3): E109–117.
Agrawal, A.A., 2017. Evolution, current status and advances in application of platelet concentrate in periodontics and implantology. World J Clin Cases; 5(5): E159–171.
Bölükbaşı, N., Yeniyol, S., Tekkesin, M.S., Altunatmaz, K., 2013. The Use of Platelet-Rich Fibrin in Combination With Biphasic Calcium Phosphate in the Treatment of Bone Defects: A Histologic and Histomorphometric Study. Curr Ther Res Clin Exp; 75: E15–21.
Borie, E., Oliví, D.G., Orsi, I.A., Garlet, K., Weber, B., Beltrán, V., Fuentes, R., 2015. Platelet-rich fibrin application in dentistry: a literature review. Int J Clin Exp Med; 8(5): E7922–7929.
Chen, Y., Xu, J., Huang, Z., Yu, M., Zhang, Y., Chen, H., Ma, Z., Liao, H., Hu, J., 2017. An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs). Scientific Reports; 7: E44130.
Choukroun J, Adda F, Schoeffler C, Vervelle A., 2001. An opportunity in perioimplantology 2001;42: E55–62.
Choukroun, J., Diss, A., Simonpieri, A., Girard, M.-O., Schoeffler, C., Dohan, S.L., Dohan, A.J.J., Mouhyi, J., Dohan, D.M., 2006. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology;101(3): E299–303.
Cortese, A., Pantaleo, G., Borri, A., Caggiano, M., Amato, M., 2016. Platelet-rich fibrin (PRF) in implant dentistry in combination with new bone regenerative technique in elderly patients. International Journal of Surgery Case Reports 28: E52.
Dohan David M., Joseph Choukroun, Antoine Diss, Steve L. Dohan, Anthony J. J. Dohan, Jaafar Mouhyi, and Bruno Gogly. 2006. Platelet-rich ﬁbrin (PRF): A second-generation platelet concentrate.Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod;101(3): E37-44.
Dohan David M., Joseph Choukroun, Antoine Diss, Alain Simonpieri, Marie-Odile Girard, Christian Schoeffler, Steve L. Dohand, Anthony J.J. Dohane and Jaafar Mouhyi, 2006. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology;101(3): E299-303.
Jang, B.J., Byeon, Y.E., Lim, J.H., Ryu, H.H., Kim, W.H., Koyama, Y., Kikuchi, M., Kang, K.S., Kweon, O.K., 2008. Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs. J Vet Sci; 9(4): E387–393.
Kim, J.-W., Shin, Y.C., Lee, J.-J., Bae, E.-B., Jeon, Y.-C., Jeong, C.-M., Yun, M.-J., Lee, S.-H., Han, D.-W., Huh, J.-B., 2017. The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis. Int J Mol Sci; 18(8): E 387-393.
Kökdere, N.N., Baykul, T., Findik, Y., 2015. The use of platelet-rich fibrin (PRF) and PRF-mixed particulated autogenous bone graft in the treatment of bone defects: An experimental and histomorphometrical study. Dent Res J (Isfahan); 12(5): E418–424.
Kumar, P., Vinitha, B., Fathima, G., 2013. Bone grafts in dentistry. J Pharm Bioallied Sci; 5(1): E125–127.
Lee, E.-U., Kim, D.-J., Lim, H.-C., Lee, J.-S., Jung, U.-W., Choi, S.-H., 2015. Comparative evaluation of biphasic calcium phosphate and biphasic calcium phosphate collagen composite on osteoconductive potency in rabbit calvarial defect. Biomater Res; 19(1).
Li, X., Yao, J., Wu, J., Du, X., Jing, W., Liu, L., 2018. Roles of PRF and IGF-1 in promoting alveolar osteoblast growth and proliferation and molecular mechanism. Int J Clin Exp Pathol; 11(7): E3294-3301
Lucaciu, O., Gheban, D., Soriţau, O., Băciuţ, M., Câmpian, R.S., Băciuţ, G., 2015. Comparative assessment of bone regeneration by histometry and a histological scoring system / Evaluarea comparativă a regenerării osoase utilizând histometria și un scor de vindecare histologică. Romanian Review of Laboratory Medicine; 23(1): E31-45.
Öncü, E., Bayram, B., Kantarcı, A., Gülsever, S., Alaaddinoğlu, E.-E., 2016. Posıtıve effect of platelet rich fibrin on osseointegration. Med Oral Patol Oral Cir Bucal; 21(5): E601–607.
Rady, D., Mubarak, R., Abdel Moneim, R.A., 2018. Healing capacity of bone marrow mesenchymal stem cells versus platelet-rich fibrin in tibial bone defects of albino rats: an in vivo study. F1000Research; 7: 1573.
Song, Y., Lin, K., He, S., Wang, C., Zhang, S., Li, D., Wang, J., Cao, T., Bi, L., Pei, G., 2018. Nano-biphasic calcium phosphate/polyvinyl alcohol composites with enhanced bioactivity for bone repair via low-temperature three-dimensional printing and loading with platelet-rich fibrin. Int J Nanomedicine; 13: E505–523.
Wang, X., Li, G., Guo, J., Yang, L., Liu, Y., Sun, Q., Li, R., Yu, W., 2017. Hybrid composites of mesenchymal stem cell sheets, hydroxyapatite, and platelet-rich fibrin granules for bone regeneration in a rabbit calvarial critical-size defect model. Exp Ther Med; 13(5): E1891–1899.
Yilmaz, D., Dogan, N., Ozkan, A., Sencimen, M., Ora, B.E., Mutlu, I., Yilmaz, D., Dogan, N., Ozkan, A., Sencimen, M., Ora, B.E., Mutlu, I., 2014. Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing. A histological study in pigs. Acta Cirurgica Brasileira; 29(1): E59–65.
Zhang, Y., Tangl, S., Huber, C.D., Lin, Y., Qiu, L., Rausch-Fan, X., 2012. Effects of Choukroun’s platelet-rich fibrin on bone regeneration in combination with deproteinized bovine bone mineral in maxillary sinus augmentation: A histological and histomorphometric study. Journal of Cranio-Maxillofacial Surgery; 40(4): E321–328.