ROOTING OF THE PURPLE ROBE Robinia pseudoacasia L. CUTTINGS AS INFLUENCED BY CUTTING TIME, COLD STORAGE AND IBA

  • OTHMAN KAMIL AREF HAWRAMEE Dept. of Horticulture -College of Agricultural Sciences Engineering ,University of Sulaimani, Kurdistan Region-Iraq
Keywords: Purple robe, Rooting, Cutting time, Storage, IBA

Abstract

The Purple Robe fruitless cultivar of black locust Robinia pseudoacacia L. is an attractive ornamental tree that widely planted throughout the world for various uses. This study was attempted to propagate this fruitless cultivar of Robinia by hardwood cuttings; at different cutting times, cold mist storage conditions and using various concentrations of IBA.

The results showed that taking and planting cuttings on March 15th, gave the best survival percentage of rooted seedlings, which was (20.83%) compared to February 1st (8.33%) and February 22nd (12.5%).

Cuttings treated with different concentrations of IBA significantly increased success percentage (38.33, 33.33, 25.00 and 14.17%) for (2000, 1500, 1000 and 0 ppm), respectively. While the cold moist storage duration of the cuttings negatively affected on survival percentage (16.67%) for 3 weeks and (12.5%) for 6 weeks storage compared to (20.83%) for non-stored cuttings.

Furthermore, the treatment combination of previous factors had positive effects on studied characters; the heighest value of rooting rate success (62.5%), seedling height (82.67 cm), vegetation fresh weight (66.3 g), vegetation dry weight (21.26 g), and number of leaves per plant (32.33) were obtained from cuttings taken on February 22nd and treated with 2000 ppm, whereas the highest means of roots fresh and dry weights (12.71 and 3.62 g), respectively, were observed from cuttings taken on February 1st and treated with 2000 ppm.

The results recommend taking cuttings on February 22nd, and using 2000 ppm of IBA directly (without cold moist storage) before planting to enhance cuttings survival percentage and seedlings quality

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References

Alvarez, R.; Nissen S. and Sutter, E. G. (1989). Relationship between Indole-3-Acetic Acid Levels in Apple (Malus pumila Mill) rootstocks cultured in vitro and adventitious root formation in the presence of lndole-3-ButyricAcid. Journal of Plant Growth Regulation, 89 (4): 439–443.
Batzli, J. M.; Graves, W. R. and Berkum, P. (1992). Diversity among Rhizobia effective with Robinia pseudoacacia. Applied and Environmental Microbiology, 58 (7): 2137–2143.
Chhun, T.; Taketa, S.; Tsurumi, S. and Ichii, M. (2003).The effects of auxin on lateral root initiation and root gravitropism in a lateral rootless mutant Lrt1 of rice (Oryza sativa L.). Plant Growth Regulation, 39 (2): 161–170.
Cierjacks, A.; Kowarik, I.; Joshi, J.; Hempel, S.; Ristow, M.; von der Lippe, M. and Weber, E. (2013). Bio¬logical flora of the British Isles: Robinia pseudoacacia. Journal of Ecology. 101 (77): 1623–1640.
Cornu, D. (1973). Preliminary tests on the selection of endemic Douglas-fir clones (Pseudotsuga menziesii (Mirb.)(Abstract). Ann. Sci. For., 30:157–173.
Crosti, R.; Agrillo, E.; Ciccarese, L.; Guarino, R.; Paris, P. and Testi, A. (2016). Assessing escapes from short rotation plantations of the invasive tree species Robinia pseudoacacia L. in Mediter¬ranean ecosystems: a study in central Italy.iForest-Biogeosciences and Forestry, 9 (5): 822.
De Almeida, J. B. S. A. and Pivetta, K. F. L. (2002). Effect of storage of cuttings on the rooting of different cultivars of cut chrysanthemums (Dendranthema grandiflora, Tzevlev) for two different seasons. In XXVI Inter. Horticultural Congress: Elegant Sciences in Floriculture, 624: 379-384.
de Andrés, E. F.; Sánchez, F. J.; Catalán, G.; Tenorio, J. L. and Ayerbe, L. (2004). Vegetative propagation of Colutea istria Mill. from Leary stem cuttings. Agroforestry Systems, 63: 7-14.
de Andrés, E. F.; Alegre, J.; Tenorio, J. L.; Manzanares, M.; Sánchez, F. J. and Ayerbe, L. (1999). Vegetative propagation of Colutea arborescens L., a multipurpose leguminous shrub of semiarid climates. Agroforestry Systems, 46: 113-121.
De Klerk, G. J.; Ter Brugge, J. and Marinova, S. (1997). Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork 9’. Plant Cell, Tissue and Organ Culture, 49 (1): 39–44.
Edward, F. G. and Watson, D. G. (1994). Robinia pseudoacasia “Purple Robe” Black Locust. Fact Sheet ST-572.
Fowells H. A. Ed. (1965). Silvics of Forest Trees of the United States. U.S. Dept. of Agriculture, Forest Service: Washington D.C., pp.642–648.
Gyana, R. R. (2006). Effect of auxins on adventitious root development from single node cuttings of Camellia sinensis (L.) Kuntze and associated biochemical changes. Plant Growth Regulation, 48 (2): 111-117.
Hanover, J. W. and Mebrahtu T. B. (1989). Genetic improvement of black locust: A prime agroforestry species. Proc. First Conf. on Agroforestry in N. America, Aug., Guelph, Ontario, Canada.
Holey, W. D. and Farmer, R. (1951). Cold storage of carnation cuttings. Hort. Abst., 21 (2): 1880-1886.
Hossain, M. A.; Islam, M. A. and Hossain, M. M. (2004). Rooting ability of cuttings of Swietenia macrophylla King and Chukrasia velutina wight et arn as influenced by exogenous hormone. International Journal of Agriculture & Biology, 6 (3): 560–564.
https://www.digimizer.com/download.php
https://www.xlstat.com/en/download
Husen, A. (2003). Effect of IBA and NAA treatments on rooting of Rauvolfia serpentina Benth. ex Kurz shoot cuttings. Ann For 11: 88-93.
Husen, A. and Pal, M. (2007). Metabolic changes during adventitious root primordial development in Tectona grandis Linn. f. (teak) cuttings as affected by age of donor plants and auxin (IBA and NAA) treatment. New Forests, 33 (3): 309-323.
Hussain, A. and Khan, A. M. (2004). Effect of growth regulators on stem cutting of Rosa bourboniana and Rosa gruss-an-teplitz. International Journal of Agriculture & Biology, 6 (5): 931–932.
Husen, A. and V.K. Mishra (2001). Effect of IBA and NAA on vegetative propagation of Vitex negundo L. through leafy stem cuttings from hedged shoots during rainy season. Ind Perf 45: 83-87.
Kamlesh, K.; Anju, B. and Raj, D. (2007). Robinia pseudoacasia Linn. Tree and Forestry Science and Biotechnolog.y, 1 (1): 74-80.
Keresztesi, B. (1980). The black locust. Unasylva, 32 (127): 23-33.
Keresztesi, B. (1988). The Black Locust. Akadémiai Kiadó, Budapest, 1–196.
Kiran, Kaul (2008). Variation in rooting behavior of stem cuttings in relation to their origin in Taxus wallichiana Zucc. New Forests36: 217-224.
Kotis, M.; Yupsanis, T. A.; Syros, T. D. and Economou, A. S. (2009). Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia×hybrida microshoots. Biologia Plantarum, 53 (3): 530-538.
Li, G.; Xu, G.; Guo, K. and Du, S. (2014). Mapping the global potential geographical distribution of black locust (Robinia Pseudoacacia L.) using herbarium data and a maximum entropy model. Forests, 5: 2773–2792.
Lopez, R. G. and Runkle, E. S. (2008). Low-temperature storage influences morphological and physiological characteristics of non-rooted cuttings of New Guinea impatiens (Impatiens hawkeri). Postharvest Biology and Technology, 50 (1): 95-102.
Marks, T.R. and S.E. Simpson (2000). Interaction of explants type and indole-3-butyric acid during rooting in vitro in a range of difficult and easy-to-root woody plants. Plant Cell, Tissue and Organ Culture 62: 65-74
Martin, K. P. (2002). Rapid propagation of Holostemma ada-kodien Sehult., a rare medicinal plant, through axillary bud multiplication and indirect organogenesis. Plant Cell Reports, 21 (2): 112–117.
Muthoo, M. K. and Kango, G. H. (1965). in India with special reference to Jammu and Kashmir. Indian Forester, 91 (2): 126-130.
Nordstr¨om, A. C.; Jacobs, F. A. and Efiasson, L. (1991). Effect of exogenous indole-3-acetic acid and indole-3-butyric acid on internal levels of the respective auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings. Plant Physiology, 96 (3): 856–861.
Ozel, C. A.; Khawar, K. M.; Mirici, S.; Arslan, O. and Ozcan, S. (2006). Induction of Ex Vitro adventitious roots on soft wood cuttings of Centaurea tchihatcheffii tchihatcheffii Fisch et. Mey using Indole 3-butyric acid and
Published
2020-07-11
How to Cite
HAWRAMEE, O. K. A. (2020). ROOTING OF THE PURPLE ROBE Robinia pseudoacasia L. CUTTINGS AS INFLUENCED BY CUTTING TIME, COLD STORAGE AND IBA. Journal of Duhok University, 22(s2), 131-142. https://doi.org/10.26682/cajuod.2020.22.2.15