@article{ALNAKSHABANDI_MERZA_KHALED_S. JUBRAEL_2020, title={STUDY GENETIC RELATIONSHIP AMONG ENTEROCOCCUS FAECALIS STRAINS COLLECTED FROM URINE HARBORED DIFFERENT VIRULENCE PROFILES USING ERIC-PCR ASSAY}, volume={23}, url={https://journal.uod.ac/index.php/uodjournal/article/view/619}, DOI={10.26682/sjuod.2020.23.1.7}, abstractNote={<p><strong>ABSTRACT</strong></p> <p><strong>&nbsp;&nbsp;&nbsp; </strong><strong>Twenty five isolates of <em>Enterococcus faecalis</em> have been previously identified and characterized were subjected to ERIC-PCR analysis in order to study the genetic relationship with regarding to their virulence profile. Nine virulence profiles were identified according to the presence/ absence of five virulence factors; <em>asa1,gelE, esp, cpd and ace</em></strong><strong>. Most isolates (28%) were belonged to Virprofile1 </strong><strong><em><sup>asa1</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>, esp</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>, cpd</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,ace</sup></em></strong><strong><sup>+ </sup></strong><strong>followed by Virprofile3</strong><strong><em><sup>asa1+,gelE-, esp+, cpd+,ace+</sup></em></strong><strong><em> (</em></strong><strong>20%), </strong><strong>Virprofile4 </strong><strong><em><sup>asa1</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>, esp</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>, cpd</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,ace</sup></em></strong><strong><sup>-</sup></strong> <strong>accounting 16%, while virprofiles5 </strong><strong><em><sup>asa1</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>,esp</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>,cpd</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,ace</sup></em></strong><strong><sup>+</sup></strong><strong><em>,</em></strong> <strong>virprofile6</strong><strong><sup> <em>asa1</em></sup></strong><strong><sup>-</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,esp</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,cpd</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,ace</sup></em></strong><strong><sup>+</sup></strong> <strong>and virprofile8</strong><strong><sup> <em>asa1</em></sup></strong><strong><sup>+</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>, esp</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>, cpd</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,ace</sup></em></strong><strong><sup>-</sup></strong> <strong>representing </strong><strong>8%, v</strong><strong>irprofile2</strong><strong><em><sup> asa1</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>,esp</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>, cpd</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>, ace</sup></em></strong><strong><sup>+</sup></strong><strong><em>, </em></strong><strong>virprofile7</strong><strong><em> <sup>asa1</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>, esp</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>, cpd</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,ace</sup></em></strong><strong><sup>-</sup></strong> <strong><em>&nbsp;</em></strong><strong>and virprofile9</strong><strong><em><sup> asa1</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,gelE</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>, esp</sup></em></strong><strong><sup>-</sup></strong><strong><em><sup>, cpd</sup></em></strong><strong><sup>+</sup></strong><strong><em><sup>,ace</sup></em></strong><strong><sup>+</sup></strong> <strong>were</strong><strong> &nbsp;4%<em>. </em></strong><strong>ERIC-PCR analysis divided isolates into two main clusters named; cluster A accounting 28% which further classified into groups; 8% isolates were belonged to A1 and 20% were belonged to A2. Most isolates were belonged to cluster B accounting 72%. This cluster was involved two groups; six isolates (33.3%) were belonged to B1 while 66.6% of isolates were assigned as B2. However, no relationship was found between virulence profiles with phylogentic groups&nbsp; of the isolates.</strong></p> <p>&nbsp;</p> <p>&nbsp;</p&gt;}, number={1}, journal={Journal of Duhok University}, author={ALNAKSHABANDI, WASAN M. and MERZA, NARMIN SAEED and KHALED, HAVAL MOHAMMED and S. JUBRAEL, JALADET M.}, year={2020}, month={Jun.}, pages={63-68} }