TY - JOUR AU - ABDULKHALEQ SALIH, DOAA AU - M. ABDO, DR. JASSIM AU - T. SAADI, DR. ABDULRAHMAN PY - 2019/11/04 Y2 - 2024/03/28 TI - MOLECULAR IDENTIFICATION OF Salmonella Enterica FROM PATIENTS WITH DIARRHEA IN DUHOK GOVERNORATE KURDISTAN REGION / IRAQ JF - Journal of Duhok University JA - JDU VL - 22 IS - 1 SE - Pure and Engineering Sciences DO - 10.26682/sjuod.2019.22.1.16 UR - https://journal.uod.ac/index.php/uodjournal/article/view/421 SP - 148-154 AB - Background and objectives: Salmonella enterica are responsible for causing the highest number of bacterial foodborne infections in the world. Methods: The Present study was carried out for detection and molecular identification of Salmonella enterica isolated from stool specimens of patients suffering from diarrhoea using traditional method and identification by using of RT-Polymerase Chain Reaction (PCR) techniques. Results: Out of the 121 patients with diarrhoea attended Azadi and Heevi Teaching Hospitals, 72 cases (59.504%) gave positive results for culturing. While by PCR method, 68 cases (56.198%) gave positive results for Salmonella enterica. In this study, (49) cases (40.495%) of non-Salmonella spp. gave negative results by culture and (53) cases (43.8%) of non-Salmonella spp. gave negative results by PCR method. Conclusions: The current study showed that RT-PCR technique is more accurate and sensitive compared to traditional methods.  ER -