TY - JOUR AU - UoD, Journal of Duhok University AU - ABDULJALIL, ZULAYKHA ABDULWAHAB AU - KASSIM, NABEEL AZIZ AU - DUHOKY, MOSLEH MOHAMMAD SAEED PY - 2020/06/01 Y2 - 2024/03/29 TI - FIRST RECORD OF DAHLIA MOSAIC VIRUS IN IRAQ ON DAHLIA (Dahlia pinnata). JF - Journal of Duhok University JA - JDU VL - 22 IS - 2 SE - Agriculture and Veterinary Science DO - 10.26682/ajuod.2019.22.2.12 UR - https://journal.uod.ac/index.php/uodjournal/article/view/558 SP - 113-120 AB - The presence of Dahlia mosaic virus (DMV) was confirmed for the first time on both leaves and tubers of Dahlia pinnata in Iraq. DMV was confirmed by amplification of opening reading frame 1 (ORF1) that is encoding for the movement protein, using the specific primer (DMV F1 and DMV R1). The expected size of about 900 bps was amplified from 30 samples. There was no evidence of infection with DMV-D10 and dahlia common mosaic virus. Mechanical and vector transmission studied as biological properties of the virus. D. pinnata, Zinnia elegans, Ageratum conyzoides cv. Blue Ball, Amaranthus caudates, Helianthus annuus and Chrysanthemum indicum were used as indicator plants. DMV was mechanically transmissible to all tested plants, at a rate of about 80%. The diseased dahlia was tested for the presence of viral infections with, TSV, TRV, CMV and TSWV using Chenopodium quinoa and Nicotiana tabacum as diagnostic species. Both species remained symptomless after several inoculations. DMV was transmitted by Myzus persicae and Aphis fabae at the rate of 60 and 55 %, respectively ER -