MOLECULAR CHARACTERAZATİON OF VİRULENCE FACTORS AMONG ANTİBACTERİAL RESİSTANT Pseudomonas Aerugınosa ISOLATED FROM BURN INFECTİONS FROM DUHOK AND ERBİL HOSPİTALS/IRAQ

  • MARWAN KHALIL QADER
  • HASAN SOLMAZ
  • NARMIN SAEED MERZA
Keywords: Burn infection, , P. aeruginosa, PCR, 16srDNA, Virulence genes

Abstract

In the current study, 225 isolates of P. aeruginosaburn infection have been collected from major hospitals in Duhok and Erbil / Iraq, from April 2015 till to September 2015. One hundred thirty six of these were isolated from males accounting 60.4 % whereas 89(39.6%) of them were recovered from females. One hundred of these  isolates were selected (fifty from each province including; Erbil and Duhok Province)  and subjected to 16 different antibiotics using disc diffusion method showed high resistancy to most tested antibiotics, 90% of these isolates were multidrug resistant, Imipeneme was considered as the most effective antibiotic effect on these isolates with resistant rate 47%.The genome all of these isolates were successfully amplified producing a single band of the 16srDNA locus in all strains with a molecular weight of about 956 bp in order to confirm at molecular level that all these isolates were P. aeruginosa.The results of the detection of five virulence related genes including (opr-1, tox-A,exo-S,las-B, and nan-1) revealed that ten of these isolates accounting (10%) lacked any tested virulence markers,oprIas a marker for presence of pathogenicity island was the most predominant marker among all other virulence markers accounting 90(90%) followed by tox-A, and exo-S accounting 86(86%) and 86(86%) respectively, while the prevalence of las-Bgene is found with the rate 82(82%) and nan-1with35(35%)respectively.

 

Downloads

Download data is not yet available.

References

Wisplinghoff H, Seifert H. 2016 Pseudomonas aeruginosa, Acinetobacter baumannii and Other Non-Fermenting Gram-Negative Bacilli. In: Infectious Diseases. (4th Edition),. Cohen, Powderly & Opal (Eds.).
Cevahir, N., Demir, M., Kaleli, I., Gurbuz, M., Tikvesli, S., 2008. Evalution of biofilm production, gelatinase activity, and mannose-resistent hemagglutination Acinetobacter baumannii strains. Journal of Microbiology and Immunolgical Infection,41:513-518
Cheesbrough, M., 2006. District Laboratory Practice in Tropical Countries, part2, 2nd Ed.Cambridge University,13: 511-978
Clinical and Laboratory Standards Institute CLSI. 2007. Performance standards for antimicrobial susceptibility testing; seventeenth informational supplement.
Antônio, J.R., Mario, R.O.B., Renan, R.R., Maria, V.L., Francisco, L.L.M and S,L.R. (2019). Pseudomonas Aeruginosa: Virulence Factors and Antibiotic Resistance Genes Vol.62:
David, A.S., Wook, C., Freshteh, T., William, E.B., 2007. Comparative global transcription analysis of sodium hypochlorite, peracetic acid, and hydrogen peroxide on P. aeruginosa. Applied Microbiology and Biotechnology, 76:1093-1105.
De Vos, D., Lim, A.J.R., Vos, P.D.E., A. Sarniguet, A., Kersters, K., Cornelis, P., 1993. Detection of the outer membrane lipoprotein I and its gene in fluorescent and non fluorescent pseudomonads: implications for taxonomy and diagnosis. Journal of General Microbiology,139:2215-2223.
Douglas, M.W., Mulholland, K., Denyer, V. and Gottlieb, T. 2001. Multi-drug resistant P.aeruginosa outbreak in a burns unit an infection control study. Burns, 27(2): 131-135.
Edwards, V. Greenwood, J. 2003. What’s new in burn microbiology? James Laing Memorial Prize Essay 2000. Burns,29(1): 15-24.
Fazeli, N., Momtaz, H., 2014. Virulence Gene Profiles of Multidrug-Resistant P. aeruginosa Isolated From Iranian Hospital Infections, Iran Red Crescent Medical Journal,16 (10):e15722.
Flores, J., Okhuysen, P.C., 2009. Enteroaggregative Escherichia coli infection. J. Current Opinion in Gastroenterol,25 (1):8-11
Gallagher, L.A., Manoil, C., 2001. P. aeruginosa PAO1 kills Caenorhabditis elegans by cyanide poisoning. Journal of Bacteriology, 183: 6207-6214.
Hancock, R.E., 1998. Resistance mechanisms in P. aeruginosa and other nonfermentative gram-negative bacteria. Journal ofClinical Infection Disease27 (1):93-99.
Hodiwala, A.; Dhoke, R., and Urhekar, A. D. 2013. Incidence of Metalo-β- Lactamse producing Pseudomonas, Acinetobacter and Enterobacterial isolates in Hospitalized Patients. International Journal of Pharma and Bio Science, 3(1):79-83.
Karimian, A., Momtaz, H., and Madani, M., 2012. Detection of uropathogenic E.coli virulence factors in patients with urinary tract infections in Iran. African Journal of Microbiology Research,6 (39): 6811-6816.
Khattab, M.A., Nour, M.S., ElSheshtawy, N.M., 2015. Genetic Identification of P. aeruginosa Virulence Genes among Different Isolates, Journal of Microbial and Biochemical Technology ,7 (5):274-277.
Lari, A.K., Bahrami, H.H. and Alaghehbandan, R. (1998). Pseudomonas infection in Tohid Burn Centre, Iran. Burns,24(7): 637-641.
Maniatis, T., Fritsch, E.F.,Sambrook, J., 1982. Molecular cloning: A Laboratory Manual Gold Spring Harber Laboratory, New York. Biotechnology,5: 257-261
Naqvi, Z.A., Hashmi, K., Rizwan, Q.M, Kharal, S.A., 2005. Multi drug resistant P. aeruginosa: A nosocomial infection threat in burn patients. Pakistan Journal of Pharmacology, 22(2):9-15.
Neely, A.N. Holder, I.A. 1999. Antimicrobial resistance. Burns, 25(1): 17-24.
Nicolle, L.E., 2005. Complicated urinary tract infection in adults. Can Jornal Infection Disease Medical Microbiology,16 (6): 349-360.
Oralancul, Yuksel, F., Altunay, H., Acikel, C.,Celikoz, B. Cavulu, A., 2002. The evaluation of nasocomial infection during 1st year period in the burn unit of a training hospital in Istanbul, Turkey. Burns, 28(8): 738-744.
Paterson, D. L., 2006. Resistance in gram-negative bacteria: Enterobacteriaceae, Journal of Academic., 34(5): 20-28.
Rawya, I., Badr, Magda, el. N., Amr el.S., Ahmed B.el.D., 2008. P. aeruginosa Exotoxin A as a Virulence Factor in Burn Wound Infections. Egyptian Journal of Medical Microbiology,17(1): 125-133.
Sadikot, R.T., Blackwell, T.S., Christman, J.W., Prince, A.S., 2005. Pathogen-host interactions in P. aeruginosa pneumonia. American Journal of Respiratory and Critical Care Medicine,171: 1209-1223
Song, W., Lee, K.M., Kang, H.J., Shin, D.H. Kim, D.K., 2001. Microbiologic aspects of predominant bacteria isolated from the burn patients in Korea. Burns,27(2): 136-139.
Spilker, T., Tom, C., Peter,V., John, J.L., 2004. PCR-Based Assay for Differentiation of P. aeruginosa from Other Pseudomonas Species Recovered from Cystic Fibrosis Patients. Journal of Clinical Microbiology, 42(5): 2074-2079.
Stover, C.K., Pham, X.Q., Erwin, A.L., Mizoguchi, S.D., Warrener, P., Hickey M.J., Brinkman, F.S.L., Hufnagle, W.O., Kowalik, D. J., Lagrou, M., Garber, R. L., Goltry, L., Tolentino, E., Westbrock-Wadman, S., Yuan, Y., Brody L.L., Coulter S. N., Folger K.R., Kas A., Larbig K., Lim R., Smith K., Spencer D., Wong G. K.-S., Wu, Z., Paulsenk, I.T., Reizer, J., Saier, M.H., Hancock, R.E.W., Lory, S. Olson, M.V., 2000. Complete genome sequence of P. aeruginosa PAO1, an opportunistic pathogen. Nature.,406:959-964
Theodore S., Tom C., Peter V., and John J. L. 2004. PCR-Based Assay for Differentiation of P. aeruginosa from other Pseudomonas Species Recovered from Cystic Fibrosis Patients. Journal of Clinical Microbiology, 42(5): 2074-2079.
Todar, K., 2009. The mechanisms of bacterial pathogenicity. Todar's Online Textbook of Bacteriology The Microbial Word Pathogenesis,8:234-243
Vincent, J.L., Chierego, M., Struelens, M., Byl, B., 2004. Infection control in the intensive care unit.Expert Review of Anti-Infective Therapy Journal,2:795-805
Wolska, K., Szweda, P., 2009. Genetic features of clinical P. aeruginosa strain., Journal of Microbiology,85(3):255-260.
Yasufuku, T.; Shigemura, K.; Shirakawa, T.; Matsumoto, M.; Nakano, Y.; Tanaka, K.; Arakawa, S.; Kinoshita, S.; Kawabata, M., Fujisawa, M. (2011). Correlation of overexpression of efflux pump genes with antibiotic resistance in Escherichia coli Strains clinically isolated from urinary tract infection patients. Journal of Clinical Microbiology, 49(1):189-94.
Published
2021-04-05
How to Cite
QADER, M. K., SOLMAZ, H., & MERZA, N. S. (2021). MOLECULAR CHARACTERAZATİON OF VİRULENCE FACTORS AMONG ANTİBACTERİAL RESİSTANT Pseudomonas Aerugınosa ISOLATED FROM BURN INFECTİONS FROM DUHOK AND ERBİL HOSPİTALS/IRAQ. Journal of Duhok University, 24(1), 1-9. https://doi.org/10.26682/sjuod.2021.24.1.1
Section
Pure and Engineering Sciences