ACCURACY OF SEROLOGICAL AND STOOL ANTIGEN TESTS (NON-INVASIVE) FOR DETECTION OF H. pylori

  • MEETHAQ M. NEAMAH AL-HILFI
  • KHAIRALLAH A.S MOHAMMED
  • ALI DAWOOD AL-HILFI
  • Naael H. Ali
Keywords: H. pylori, H. pylori stool Ag, Serological tests, Rapid urease test

Abstract

Background  Helicobacter pylori is a major bacterial causative pathogen of various gasto-doudenal diseases, including chronic gastritis, peptic ulcer disease (PUD), gastric cancer, and mucosa-associated lymphoid tissue lymphoma. The prevalence of H. pylori is about 50% worldwide and could reach more than 70% in developing countries. The aim of the present study was to  evaluate the diagnostic accuracy of both serological and stool antigen tests comparing  with rapid urease test for detection of H. pylori.

Methods A 114 patients with gastroduodenal disorders aged 20- 60 years were   recruited in this study. Endoscopic biopsy was obtained from each patient at endoscopic unite in the Al Sader Hospital, Basrah/Iraq. All biopsies were used for rapid urease test. Three ml. of venous blood were collected from each patient and used for serological test.  Stool samples were collected and used for detection of H.pylori Ag. The sensitivity, specificity, positive predictive value, negative predictive value, accuracy, and area of these under receiver operating characteristic (ROC) curve for detection H.pylori Ag/antibody tests were determined using rapid urease test as gold standard method.

Result The results showed that the sensitivity of H. pylori Ag and serological test   were (92.6%, 69%), specificity (69.9%, 63.6%), positive predictive value (98.6 %, 82%)  negative predictive value (84%, 45.6%) accuracy (93.8%, 67%) and area under ROS (0.9-0.996, 0.664-0.776) respectively.

Conclusion: Stool antigen test is more reliable test for detection H. pylori in comparison to rapid urease test.

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References

Smith S, Fowora M, Pellicano R. Infections with Helicobacter pylori and challenges encountered in Africa. World J Gastroenterol. 2019;25(25):3183.
Wang Y-K, Kuo F-C, Liu C-J, et al. Diagnosis of Helicobacter pylori infection: Current options and developments. World J Gastroenterol WJG. 2015;21(40):11221.
Pereira V, Abraham P, Nallapeta S, Shetty A. Gastric bacterial Flora in patients Harbouring Helicobacter pylori with or without chronic dyspepsia: analysis with matrix-assisted laser desorption ionization time-of-flight mass spectroscopy. BMC Gastroenterol. 2018;18(1):1-8.
Abboud AA, MouAssawi HA, Rustom M, Khalek WA. Epidemiology of Helicobacter pylori infection among symptomatic patients, correlation with endoscopic findings and it’s association with type II diabetes mellitus. J Gastroint Dig Syst. 2017;7(3):1-5.
Al-Mashhadany DA, Ismael LQ, Zaki AM. Seroprevalence of Helicobacter pylori among human in Erbil governorate, Kurdistan region, Iraq. Res J Life Bioinform Pharm Chem Sci. 2018;4(2):268-280.
Perrais M, Rousseaux C, Ducourouble M-P, et al. Helicobacter pylori urease and flagellin alter mucin gene expression in human gastric cancer cells. Gastric Cancer. 2014;17(2):235-246.
Carlini CR, Ligabue-Braun R. Ureases as multifunctional toxic proteins: a review. Toxicon. 2016;110:90-109.
KAazemi S, Tavakkoli H, Habizadeh MR, Emami MH. Diagnostic values of Helicobacter pylori diagnostic tests: stool antigen test, urea breath test, rapid urease test, serology and histology. J Res Med Sci Off J Isfahan Univ Med Sci. 2011;16(9):1097.
de Brito BB, da Silva FAF, Soares AS, et al. Pathogenesis and clinical management of Helicobacter pylori gastric infection. World J Gastroenterol. 2019;25(37):5578.
Frenck RW, Fathy HM, Sherif M, et al. Sensitivity and specificity of various tests for the diagnosis of Helicobacter pylori in Egyptian children. Pediatrics. 2006;118(4):e1195-e1202.
Graham DY, Miftahussurur M. Helicobacter pylori urease for diagnosis of Helicobacter pylori infection: A mini review. J Adv Res. 2018;13:51-57.
Chey WD, Wong BCY, Gastroenterology PPC of the AC of. American College of Gastroenterology guideline on the management of Helicobacter pylori infection. Am J Gastroenterol. 2007;102(8):1808-1825.
Lee Y-C, Tseng P-H, Liou J-M, et al. Performance of a one-step fecal sample-based test for diagnosis of Helicobacter pylori infection in primary care and mass screening settings. J Formos Med Assoc. 2014;113(12):899-907.
Choi J, Kim CH, Kim D, et al. Prospective evaluation of a new stool antigen test for the detection of Helicobacter pylori, in comparison with histology, rapid urease test, 13C‐urea breath test, and serology. J Gastroenterol Hepatol. 2011;26(6):1053-1059.
Sabbagh P, Mohammadnia-Afrouzi M, Javanian M, et al. Diagnostic methods for Helicobacter pylori infection: ideals, options, and limitations. Eur J Clin Microbiol Infect Dis. 2019;38(1):55-66.
Ahn HJ, Lee DS. Helicobacter pylori in gastric carcinogenesis. World J Gastrointest Oncol. 2015;7(12):455.
Miftahussurur M, Yamaoka Y. Helicobacter pylori virulence genes and host genetic polymorphisms as risk factors for peptic ulcer disease. Expert Rev Gastroenterol Hepatol. 2015;9(12):1535-1547.
Hussen BM, Qader SS, Ahmed HF, Ahmed SH. The prevalence of Helicobacter pylori among university students in Iraq. Indian J Sci Technol. 2013;6(8):5019-5023.
Lee JY, Kim N. Diagnosis of Helicobacter pylori by invasive test: histology. Ann Transl Med. 2015;3(1).
Calvet X, Sánchez-Delgado J, Montserrat A, et al. Accuracy of diagnostic tests for Helicobacter pylori: a reappraisal. Clin Infect Dis. 2009;48(10):1385-1391.
Published
2021-04-05
How to Cite
AL-HILFI, M. M. N., MOHAMMED, K. A., AL-HILFI, A. D., & Ali, N. H. (2021). ACCURACY OF SEROLOGICAL AND STOOL ANTIGEN TESTS (NON-INVASIVE) FOR DETECTION OF H. pylori. Journal of Duhok University, 24(1), 54-59. https://doi.org/10.26682/sjuod.2021.24.1.8
Section
Pure and Engineering Sciences