ISOLATION AND CHARACTERIZATION OF Isaria farinosa FROM IRAQI GARA MOUNTAIN SOIL USING MORPHOLOGICAL AND MOLECULAR APPROACHES
Isaria farinosa, formerly known as Paecilomyces farinosus, have a worldwide distribution and a relatively wide host range. The aim of the work is to isolate and identify Isaria fungus from soil of Gara mountain using molecular analysis and morphological characteristics Isaria species were isolated from soil samples collected at an insect overwintering sites in Gara mountain, Kurdistan Region, Iraq using selective medium as an Oat culture modified with CTAB and cyclohexamide. Results demonstrated that conidia of the isolated species are globose to subglobose to ellipsoidal in shape, 2-2.3 x 1-1.3 um in size, and hyaline with smooth walls. Besides, PCR based method revealed that the sequences of the isolated fungus high level (100%) of sequence homology to the fungus I. farinosa. The present study provides better insight into the Isaria species and related entomopathogenic fungi in terms of isolation, morphology and phylogeny
Ali-Shtayeh MS, Mara’I ABBM, Jamous RM (2002). Distribution, Occurrence and Characterization of Entomopathogenic Fungi in Agricultural Soil in the Palestinian Area, Mycopathologia, 156, 235-244.
ARSEF Collection of Entomopathogenic Cultures (2021) U.S. Department of Agriculture, Agricultural Research Services. Ithaca, New York.
Assaf LH (2007) Ecological study and evaluation of activity of Beauvaria bassiana (Bals.) Vuill and Paecilomyces farinosus (Dicks ex Fr.) on some biological aspects of sunn pest on wheat (Ph.D thesis). University of Mosul, College of Agriculture and Forestry, Iraq, 231pp. (In Arabic).
Assaf LH, Haleem RA, Abdullah SK (2011) Association of entomopathogenic and other opportunistic fungi with insects in dormant locations. Jordan Journal of Biological Sciences 4:87-92.
Da Silva-Lopez R, De Oliveria LG, Lima GD, Dos Santos Correia MT, da Costa AF, De Luna-Alves Lima EA, De Menezes Lima UL (2019) Pathogenic activity of Isaria spp. For control of Dactylopsis opuntiae (Hemiptera: Dactylopiideae) and the effects of selected insecticides. Acta Zoologica Mexicanica 35, e35022449.
Domsch KH, Gams W, Anderson TH (1980) Compendium of Soil Fungi, London: Academic Press, pp. 136-140.
Gallou A, Serna-Dominguez MG, Berlanga-Padilla AM et al (2016) Clarification of Isaria isolates used as biocontrol agents against Diaphorina citri (Hemiptera: Liviidae) in Mexico. Fungal Biology 120 (3), 414-423.
Gams W, Hodge KT, Samson RA, Korf RP, Seifert KA (2005) Proposal to Conserve the Name Isaria (anamorphic fungi) with a Conserved Type, Taxon, 54, 537.
Hassan FR, Abdullah SK, Assaf LH (2019) First record of Beauveria varroae from Iraq. Nova Hedwigia 108: 427-433.
Hassan FR, Abdullah SK, Assaf LH (2020) Beauveria pseudobassiana Rehner And Humber, 2011 A New Entomopathogenic Fungus from Gara Mountain, Iraq. The Journal of Animal & Plant Sciences, 30(6): 1574-1578.
Hassan WA, Assaf LH, Abdullah SK (2012) Occurrence of entomopathogenic and other opportunistic fungi in soil collected from insect hibernation sites and evaluation of their entomopathogenic potential. Bull. Iraq nat.Hist.Mus.12:19-27.
Kleespies R, Bathon H, Zimmermann G (1989) ‘Untersuchungen zum natu¨rlichen Vorkommen von entomopathogenen Pilzen und Nematoden in verschiedenen Bo¨den in der Umgebung von Darmstadt’, Gesunde Pflanzen, 41, 350-355.
Minarni EW, Soesanto L, Suyanto A, Rostaman (2021) Molecular identification of three entomopathogenic fungi infecting the brown plant hopper pest in Indonesia. Egyptian Journal of Biological Pest Control 31:62
Niu X, Xie W, Zhang J, Hu Q (2019) Biodiversity of Entomopathogenic fungi in the soils of South China. Microorganisms 7,311; doi:10.3390/microorganisms 7090311
Posadas JB, Comerio RM, Mini JI, Nussenbaum AL, Lecuona RE (2012) A novel dodine-free selective medium based on the use of cetyltrimethyl ammonium bromide (CTAB) to isolate Beauveria bassiana, Metarhizium anisopliae sensolato and Paecilomyces lilacinus from soil. Mycologia .104:974-980.
Samson RA (1974) ‘Paecilomyces and Some Allied Hyphomycetes’, Studies in Mycology, 6, 32-36, 38-41.
Shin TY, Choi JB, Bae SM, Koo HN, Woo SD (2010) Study on selective media for isolation of entomopathogenic fungi. Int.J. Indust.Entomol.20(1):7-12.
Shreshtha B, Sung GH, Sung JM (2017) Current nomenclatural changes in Cordyceps Sensu lato and its multidisciplinary impacts. Mycology 8(4): 293-302 https://doi.org/10.1080/21501203.2017.1386242.
Sung GH, Hywel-Jones NL, Sung JM, Luangsa-ard JJ, Shrestha B, Spatafora JW (2007) ‘Phylogenetic Classification of Cordyceps and the Clavicipitaceous Fungi’, Studies in Mycology, 57, 5-59.
White T, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Inn; Innis, M., Gelfond, D., Shinsky, I and White, T.(eds.): PCR protocols: A guide to methods and applications; p.315-322.Academic Press, New York.
Zimmermann G (1986) ‘The ‘‘Galleria Bait Method’’ for Detection of Entomopathogenic Fungi in Soil’, Journal of Applied Entomology, 102, 213-215.
Zimmermann G (2008) The entomopathogenic fungi Isaria farinosus (formerly Paecilomyces farinosus) and Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocontrol Science and Technology 18;865-890. http://doi.org/10.1080/09583150802471812
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