NASAL CARRIAGE OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS) MRSA) AMONG MEDICAL AND NON-MEDICAL STUDENTS  OF SHEIKHAN-POLYTECHNIQUE COLLEGE AND  TECHNICAL INSTITUTE, IRAQ

NAJIM A  YASSIN

doi:10.26682/sjuod.2022.25.2.27

NAJIM A YASSIN Dept. of Medical Microbiology, College of Medicine, University of Duhok, Kurdistan Region-Iraq

DOI: https://doi.org/10.26682/sjuod.2022.25.2.27

Keywords: MRSA, Methicillin Resistant Staphylococcus aureus, Nasal swab, mecA gene, Medical and non-students

Abstract

Background and Aims In population, Staphylococcus aureus is present in the nasal vestibule of 35%-50% and one tenth of these are methicillin resistant S aureus (MRSA). This study aimed to investigates the prevalence of MRSA nasal carriage among students of Sheikhan-Polytechnique College (Public health and Medical laboratory technology Department) and Technical Institute (TI). Methods: During December 2018 to February 2019 data collection has been achieved. About 150 students are screened, 66 were males and 84 females with age ranged (18-24 years). A cross-sectional study was conducted and 150 nasal swabs were taken from students groups of several grades in various departments (Public Health, Medical laboratory technology and IT department). The samples were examined through standard microbiological methods. Antibiotic susceptibility tests for all isolates were checked for methicillin resistance using disk diffusion agar. Molecular characterization using PCR test targeting mecA gene was studied. Results: The overall prevalence of MRSA was 27(18%), (12 males and 15 females). High prevalence found among public health students 12/27(44.4%) followed by information technology 9/27(33.3%) and less 6/27(22.2) cases in laboratory technology students. Students of first grade followed by second grade of various departments recorded high rates than third and fourth grade (12, 9, 3 and 3, respectively). Moreover, MRSA was higher among those students that live within their family 19/27(70.3%) than those that live in hostels 8/27(29.6%) and negative in those that live with family relatives. Out of 28 MRSA isolate 14(51.8%) were positive for mecA gene and public health students accounted more carriage 8/14 (57.1%). Conclusions: This study highlighted that students of several grades of medical study are more carriers and should admit enough information concerning control measures to limit contaminate with this pathogen

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References

Ansari, S., Gautam, R., Shrestha S., Ansari, S. R., Subedi, S. N ., Chhetri, M. R . Risk factors assessment for nasal colonization of Staphylococcus aureus and its methicillin resistant strains among pre‑clinical medical students of Nepal . BMC Res Notes. 2016 ; 9(214) : 2-8.
Armstrong-Esther, C. A. Carriage patterns of Staphylococcus aureus in a healthy non-hospital population of adults and children. Ann Hum Biol. 1976; 3(3): 221–7.
Asciak, P. P., Bhimrao, S . K., Kozak, F. K., Westerberg, B. D . Health care professionals’ neckties as a source of transmission of bacteria to patients: A systematic review. CAMJ. 2018 ; 6: E26–E30.
Assafi, M. S., Mohammed, R. Q., Hussein, N. R . Nasal Carriage Rates of Staphylococcus aureus and CA-Methicillin Resistant Staphylococcus aureus among University Students. Journal of Microbiology Research. 2015; 5(4): 123-127.
Babb, J. R., Davies, J. G., Ayliffe, G. A . Contamination of protective and nurses uniforms in an isolation ward. J Hosp Infect. 1083 ;4: 149-57.
Baliga, S., Bansil, R., Suchitra, U., Bharati, B., Vidyalakshmi, K., Shenoy, S . Nasal carriage of meticillin-resistant Staphylococcus aureus in medical students. J Hosp Infect. 2008 ; 68: 91-92.
Bellows, C., Smith, A., Wheeler, J., Morici, L . Nasal Carriage of methicillin-resistant Staphylococcus aureus among students at a Louisiana medical University. Brazillian Journal of Infectious Diseases. 2013; 17(1): 118-9.
Bettin, A., Causil, C., Reyes, N . Molecular identification and antimicrobial susceptibility of Staphylococcus aureus nasal isolates from medical students in Cartagena, Colombia. Braz J Infect Dis. 2012; 16: 329-334.
Boost., M.V., O’donoghue., M.M., James, A., O’donoghue, J. Prevalence of Staphylococcus aureus carriage among dogs and their owners. Epidemiol Infect. 2008;136(7):953–64.
Cirkovic, I., Djukic, S., Vukovic, D., Stevanovic, G., Svabic- Vlahovic, M., Stepanovic, S . Nasal carriage of methicillin-resistant Staphylococcus aureus among medical students ofBelgrade University. SrpArhCelokLek. 2013; 141 (5-6): 349-53.
Clinical and Laboratory Standards Institute (2021). M100 Performance Standards for Antimicrobial Susceptibility Testing. 31th ed. Wayne, Pennsylvania USA.
D’Souza, M., Kotigadde, S., Saralaya,. K V., Kotian, M. S (2013). Prevalence of Methicillin Resistant Staphylococcus aureus Carriage amongst Health Care Workers of Critical Care Units in Kasturba Medical College Hospital, Mangalore, India. J Clin Diagn Res. 2013; 7(12) : 2697-700.
Debby, B. D., Leonard, A., Mermel, D. O., ScM,a,,B ., Steve, P. Methicillin-resistant Staphylococcus aureus transmission: The possible importance of unrecognized health care worker carriage. Am J Infect Control. 2008; 36: 93-7.
Efa, F., Alemu Y., Beyene,. G, Gudina, E.K., Kebede, W . Methicillin-resistant Staphylococcus aureus carriage among medical students of Jimma University, Southwest Ethiopia. Heliyon. 2019; 5: e01191.
Eveillard, M., Martin, Y., Hidri, N., Boussougant, Y., Joly- Guillou, M. L . Carriage of Methicillin-resistant Staphylococcus aureus among hospital employees: prevalence, duration, and transmission to households. Infect Control Hosp Epidemiol. 2004; 25(2):114-20.
Hogue, M. H., Heilmann, K. P., Callaghan, J J . Wearing ID Badges in the Operating Room Environment: Is Reconsideration Warranted? J Arthroplast. 2017; 32: 2231–2233.
Hussein, N., Salih, R S., Rasheed, N A. Prevalence of Methicillin-Resistant Staphylococcus aureus in Hospitals and Community in Duhok, Kurdistan Region of Iraq. Int J Infect. 2019; 6(2): e89636.
Jenkins, B. A., Diep, T. T, Mai, N. H. , Warrener, V. P., Suzich, J., Stover, C. K.., et al., . Differential expression and roles of Staphylococcus aureus virulence determinants during colonization and disease. mBio. 2015; 6 (1) : 02272-02214.
Jones, J. S., Hoerle, D., Riekse, R . Stethoscopes: A Potential Vector of Infection? Ann Emerg Med. 1995; 26:296–299.
Kateete, D.P., Bwanga, F., Seni, J., Mayanja, R., Kigozi, E., Mujuni, B., et al. CA-MRSA and HA-MRSA coexist in community and hospital settings in Uganda. Antimicrob Resist Infect Control. 2019; 8: 94.
Kitti, T., Boonyonying, K., Sitthisak, S . Prevalence of methicillin-resistance Staphylococcus aureus among university students in Thailand. Southeast Asian J Trop Med Public Health. 2011; 42: 1498-1504.
Kluytmans, J., Wertheim, H.F. L . Nasal carriage of Staphylococcus aureus and prevention of nosocomial infections. Infection. 2005; 33: 3-8.
Koneman, E.W., Allen S.D., Janda, W.M,, Schreckenberger, P.C., Winn, W.C. Color Atlas and Textbook of Diagnostic Microbiology. Philadelphia: JB Lippincott,1992.
Laub, K., Kardos, S., Nagy. K., Dobay, O . Detection of Staphylococcus aureus nasal carriage in healthy young adults from a Hungarian university. Acta Microbiol Immunol Hung. 2011; 58: 75-84.
Loh, W., Ng, V V., Holton, J. Bacterial flora on the white coats of medical students. J Hosp Infect. 2000; 45: 65-8.
Marín, L. F., Arciniegas, G. E., Vivas, M. C . Characterization of Staphylococcus aureus Isolates That Colonize Medical Students in a Hospital of the City of Cali, Colombia. International Journal of Microbiology. 2015; 1: 1- 6 .
Mat, A N., Pung, H. P., Abdul Rachman, A. R., Amin Nordin, S., Sarchio, S. N., Suhaili, Z., et al., . A persistent antimicrobial resistance pattern and limited methicillin-resistance-associated genotype in a short-term Staphylococcus aureus carriage isolated from a student population. J Infect Publ Health. 2016; 10 (2): 156e164. Sollid, J.U., Furberg, A S., Hanssen, A M., Johannessen, M (2014). Staphylococcus aureus: determinants of human carriage. Infect Genet Evol. 2016; 21: 531e541
Mohammed, S. H., Hmood,. M.N,, Abd, A A,, Obaid, S. A., Fahad, B. A., Fatemah, H., et al., Screening of nasal carriage for Staphylococcus aureus and their resistance to oxacillin and cefoxitin among medical students in Karbala University. J Contemp Med Sci. 2015; 1( 1): 13–16.
Pantosti, A., Sanchini A., Monaco, M . Mechanisms of antibiotic resistance in Staphylococcus aureu. Future Microbiology. 2007; 2:323-234.
Perry, C., Marshall, R., Jones, E . Bacterial contamination of uniforms. J Hosp Infect. 2001; 48: 238-41.
Pu, W., Su Y., Li, J., Li, C., Yang, Z., Deng, H. High incidence of oxacillin-susceptible mecA-positive Staphylococcus aureus(OS-MRSA) associated with Bovine mastitis in China. PLoS One . 2014; 9: e88134.
Rampal, S., Zainuddin, N. H,, Elias, N. A., Jamaluddin, T. Z ., Maniam, S., Teh S.W., et al., . Colonization of Methicillin-Resistant Staphylococcus aureus (MRSA) among Medical Students in Tertiary Institution in Central Malaysia. Antibiotics. 2020; 9: 1-9.
Ristagno, E. H., Bryant, K. A., Boland, L. F., Boland, G. G., Stout, A. D., Junkins, C. R., et al., . Effect of intranasal mupirocin prophylaxis on methicillin-resistant Staphylococcus aureus transmission and invasive Staphylococcal infections in a neonatal intensive care unit, Infect. Control Hosp Epidemiol. 2018; 39 (6) : 741e745.
Saeed, E. N., Al-Deen, H. I., Merdaw, M. A . Determine Nasal Carriage of Methicillin Resistant Staphylococcus aureus MRSA in Young Adult College Student. Iraqi J Pharm Sci. 2014; 23(1) : 83-88.
Sande, S ., Basak, S . White coats: How much safe are they? Int J Adv Med . 2015; 2: 16.
Sharma, S., Pal, S., Negi, V., Juyal, D., Sharma, M., Prakash R . Staphylococcus aureus including MRSA nasal carriage among hospital exposed and unexposed medical students. J Family Med Prim Care. 2020; 9: 4936-41.
SR,. V. P., D'Souza A.O., Vinod, R. Comparison of Phenotypic and Genotypic Characterization Methods for the Detection of Methicillin-Resistant Staphylococcus Aureus. Cureus. 2022; 14(3):e23396.
Vijay, C., Kiran,. P.R., Nagaraj, S., Srikanth, N. S., Rajitha, K. Are medical students and interns at risk of acquiring methicillin resistant Staphylococcus aureus (mrsa)?. National Journal of Community Medicine. 2016; 7: 573-579.
Xiao, X M. , Dan, D. S., Wang, S., Wang , M. L , Shang, M. H., et al . Nasal carriage of methicillin-resistant Staphylococcus aureus among preclinical medical students: epidemiologic and molecular characteristics of methicillin-resistant S, aureus clones. Diagn Microbiol Infect Dis. 2011; 70(1): 22-30.
Yassin, N. A., Hassan, A. O. Nasal carriage of methicillin-resistant/sensitive Staphylococcus aureus among students in Faculty of Medical Sciences, Duhok University. Adv Trop Med Pub Health Int. 2013; 3: 65 - 72.
Zakai, S.A . Prevalence of methicillin-resistant Staphylococcus aureus nasal colonization among medical students in Jeddah, Saudi Arabia. Saudi Med J. 2015; 36 (7): 807-812.
Zheng, B., Jiang, S.,, Xu, Z., Xiao, Y., Li, L. Severe infective endocarditis with systemic embolism due to community associated methicillin-resistant Staphylococcus aureus ST630. Braz J Infect Dis. 2015 ; 19 (1): 85e89