CALLUS INITIATION AND PLANT ORGAN REGENERATION OF ARBUTUS ANDRACHNE L. FROM LEAVES AND NODES GROWN FROM IN VITRO SEED CULTURE

LOLAV S.  SULAIMAN; RAFAIL S.  TOMA

doi:10.26682/ajuod.2023.26.1.8

LOLAV S. SULAIMAN Dept. of Forestry, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region-Iraq
RAFAIL S. TOMA Dept. of Horticulture, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region-Iraq

DOI: https://doi.org/10.26682/ajuod.2023.26.1.8

Keywords: Arbutus andrachne L., Callus, Nodes, Leaves, BA, TDZ, NAA, PPM (Plant preservative mixture)

Abstract

A successful attempt was done to overcome the traditional seed germination barriers of Arbutus andrachne L. by applying the tissue culture technique. Seeds were successfully disinfested by NaOCl and ethyl alcohol to produce healthy cultures. Treating with GA₃ raised the germination rate from only 17% (control) to 45.7 % and 54.28% by adding 1 mg. l^-1 GA₃ and 2.5 mg. l^-1 BA with and without cold pretreatment. As well as, the above GA₃ treatment was very beneficial in increasing the speed of seed germination. For callus induction, the culture of node explants was superior to the culture of leaf discs by reaching 100% as compared to only 66% of leaves. The cultured on WPM with both BA and TDZ respectively records the best callus induction (100%). The best-combined treatment was the culture of nodes on WPM enriched with 1.0 mg.l^-1 NAA+ 1.0 mg.l^-1 BA by producing the highest callus weight reaching 34.250 g. Finally, interesting organogenesis and regeneration were noticed on the induced callus from both leaf discs and nodes. The best shoot and root regeneration were noticed on node explants grown on WPM enriched with 1.0 mg. l^-1 NAA and 2.0 mg. l^-1 BA

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AL Sakran, M., Almhemed, K., & Ustuner, T. Study the Indicators of Germination and Growth of Some Species of Weeds under Different Thermal Conditions. International Journal of Research Studies in Agricultural Sciences (IJRSAS), 2020; 6(9), pp. 1-5. doi.org/10.20431/2454-6224.0609001.
Aljabari, Z., Alzeer, J., & Arafeh, R. (2014). Catechin detection in callus and in vitro cultures of the Eastern strawberry tree, Arbutus andrachne L., an endangered medicinal tree in Palestine. Global Journal of Research on Medicinal Plants & Indigenous Medicine, 3(5), 196.‏
Al-jabary, Z. (2011). In Vitro Propagation of Arbutus andrachne L. and the Detection of Catechin. Palestine Polytechnic University. Hebron. www.biotech.ppu.edu/sites/default/files/thesis/Zahra%20Yasser%20Al-jabary.pdf
Allan E (1991). Plant cell culture. In: Plant cell and tissue cultuer. (A. Stafford, and Warren, G. – Ed.). Chichester, Johan Wiley & Sons. PP. 1-25.
Bertsouklis, K. F., & Papafotiou, M. (2009). In vitro propagation of Arbutus andrachne L. Acta Hortic. 813:477–480. doi.org/10.17660/ActaHortic.2009.813.63
Dalla Guda, C., Giovannini, A., Liotta, A., Paterniani, T., Burchi, G., Cacini, S., & Aprile, S. (2010, August). Development of new ornamental plants and germplasm selection in Mediterranean native species. In XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on 937 (pp. 45-50). doi.org/10.17660/ActaHortic.2012.937.3
Edmondson, J. R., Mill, R. R., & Parris, B. S. (1978). ERICACEAE. In P. H. DAVIS (Ed.), Flora of Turkey and the East Aegean Islands, Volume 6 (Vol. 6, pp. 89–107). Edinburgh University Press. http://www.jstor.org/stable/10.3366/j.ctvxcrg60.10
El-Mahrouk, M., Dewir, Y.H., & Omar, A.M. (2010). In Vitro Propagation of Adult Strawberry Tree (Arbutus Unedo L.) Through Adventitious Shoots and Somatic Embryogenesis. Propagation of Ornamental Plants. 10(2): 93-98. www.journal-pop.org/2010_10_2_93-98.html
Giordani, E., & Bellini, E. (2005). " In vitro" Germination of Strawberry Tree (" Arbutus unedo" L.) Genotypes: Establishment, Proliferation, Rooting and Callus Induction. " In vitro" Germination of Strawberry Tree (" Arbutus unedo" L.) Genotypes, 1000-1005.‏
ISTA. (1999). International rules for seed testing. Seed science and Technology, 21:288 pp
Karam, N. S., & Al-Salem, M. M. (2001). Breaking dormancy in Arbutus andrachna L. seeds by stratification and gibberellic acid. Seed Science and Technology. 29: 51–56.
Kose, H. (1998). Studies on the germination of some woody ornamental plants existing in Turkish flora. Anadolu J. AARI 8:55–65 [in Turkish].
Miguel, M.G., Faleiro, M.L., Guerreiro, A.C., & Antunes, M.D. (2014). Arbutus unedo L.: chemical and biological properties. Molecules 19 (10), 15799–15823. doi.org/10.3390/molecules191015799
Mostafa, S.E., Karam, N.S., Shibli, R. A., & Alali, F. Q. (2010). Micropropagation and production of arbutin in oriental strawberry tree (Arbutus andrachne L.). Plant Cell Tissue Organ Cult. 103:111–121. doi.org/10.1007/s11240-010-9760-2
Odeh, S. (2017). Factors Affecting In Vitro Direct Shoot Regeneration in the Greek Strawberry Tree, Arbutus andrachne L.Palestine Polytehnic University & Bethlehem University.‏
Olmez, Z., Temel, F., Gokturk, A., & Yahyaoglu, Z. (2007). Effect of cold stratification treatments on germination of drought tolerant shrubs seeds. Journal of Environmental Biology, 28(2), 447.
Papafotiou, M., Bertsouklis, K.F., & Trigka, M. (2013). Micropropagation of Arbutus unedo, A. andrachne, and their natural hybrid A. × andrachnoides, from seedling explants. J. Hortic. Sci. Biotechnol. 88 (6), 768–775. doi.org/10.1080/14620316.2013.11513037.
Said, O., Khalil, K. Fulder S., & Azaizeh, H. (2002). Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region. Journal of Ethnopharmacology. 83:251-265. doi.org/10.1016/S0378-8741(02)00253-2
Sakar, M. K., Berkman, M. Z., Calis, I., & Ruedi, P. (1991). Constituents of Arbutus andrachne. Fitoterapia. 62: 176-177.
ŠATOVIČ, Z. (2004). Legal protection, conservation and cultivation of medicinal and aromatic plants in Croatia. In: Report of Working Group on Medicinal and Aromatic Plants. 1st Meeting. 12-14 September 2002. Gozd Martuljek, Slovenia. International Plant Genetic Resources Institute, Rome, Italy. 34–38. https://books.google.iq/books?id=AB0t7-hFy-wC
Shahbaz, S. E. (2010). Trees and Shrubs, A field guide to the trees and shrubs of Kurdistan region of Iraq. University of Duhok Press.
Shahbaz, S. E. (2020). personal communication, professor in plant taxonomy, College of Agriculture engineering science, University of Duhok.
Tilki, F., & Güner, S. (2007). Seed germination of three provenances of Arbutus andrachne L. in response to different pretreatments, temperature and light. Propogation of Ornamental Plants. 7(4): 175-179. https://hdl.handle.net/11494/1106 .

CALLUS INITIATION AND PLANT ORGAN REGENERATION OF ARBUTUS ANDRACHNE L. FROM LEAVES AND NODES GROWN FROM IN VITRO SEED CULTURE

Abstract

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