EFFECT OF GARLIC OIL ON SOME BIOCHEMICAL PARAMETERS IN RATS EXPOSED TO HYDROGEN PEROXIDE

  • Journal of Duhok University UoD
  • AZIZA RAOF HAJI Dept. of Physiology, Anatomy, and Theriogenology, Collage Of Veterinary Medicine, University Of Duhok , Kurdistan Region-Iraq.
  • TARIK ABDULGADIR ABDAL Dept. of Physiology, Anatomy, and Theriogenology, Collage Of Veterinary Medicine, University Of Duhok , Kurdistan Region-Iraq
  • BALGEES AHMED ALI Dept. of Medicine And Surgery, Collage Of Veterinary Medicine, University Of Duhok , Kurdistan Region-Iraq.
  • MEGDAD S. AHMED Duhok Research Centre, Collage Of Veterinary Medicine, University Of Duhok, Kurdistan Region-Iraq
Keywords: lipid profile, liver parameters, garlic, antioxidant

Abstract

Introduction: Medicinal plants have therapeutic impacts in both modern and traditional medicines. Therefore, some biochemical parameters were investigated in rats fed with garlic oil daily for three weeks.

Methods: A total of 20 healthy adult Wister albino male rats aged eight weeks and older were allocated into four equal groups as follow. Group I or control treated with normal diet and water. Group II/ administrated by 1% hydrogen peroxide (H2O2) with drinking water in a dark bottle prepared daily with normal diet. Group III administrated with 5 ml garlic oil added to 25 grams of their ratio for each rat (25oil/125g) also prepared daily with normal drinking water. Group IV also administrated with 5 ml of garlic oil added to 25 gram of their ratio with drinking water that contained1% hydrogen peroxide in a dark bottle.

Results: The subjects in the garlic group had significantly lower total cholesterol (54.30 vs. 68.90 mg/dl); VLDL (12.64 vs. 14.62 mg/dl); LDL (32.27 vs. 46.75 mg/dl); and higher HDL (9.39 vs. 7.53 mg/dl)) compared to the control group. The difference in liver and kidney parameters between the control and H2O2 + garlic was not significant. Similarly, no significant difference was found between the garlic and control group in GPT (20.23 vs. 23.38); urea (40.80 vs. 37.10); uric acid (0.53 vs. 0.59); and creatinine (0.57 vs. 0.78); except for GOT (15.80 vs. 30.04).

Conclusions: The present study showed the garlic extract is effective to reduce total cholesterol, LDL, VLDL, GOT and increase HDL levels.

Downloads

Download data is not yet available.

Author Biography

Journal of Duhok University UoD

Journal of Duhok University

University of Duhok

Kurdistan Region - Iraq

References

REFERENCES

Amagase, H. Clarifying the real bioactive constituents of garlic. The Journal of nutrition. 2006; 136(3): 716S-725S

Amagase, H, Petesch, BL, Matsuura, H, Kasuga, S, & Itakura, Y. Intake of garlic and its bioactive components. The Journal of nutrition. 2001; 131(3): 955S-962S

Banerjee, S, Mukherjee, PK, & Maulik, S. Garlic as an antioxidant: the good, the bad and the ugly. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2003; 17(2): 97-106

Banerjee, SK, & Maulik, SK. Effect of garlic on cardiovascular disorders: a review. Nutrition journal. 2002; 1(1): 4

Block, E. The chemistry of garlic and onions. Sci Am. 1985; 252(3): 114-119

Chen, L, Hong, JY, So, E, Hussin, AH, Cheng, WF, & Yang, CS. Decrease of hepatic catalase level by treatment with diallyl sulfide and garlic homogenates in rats and mice. Journal of biochemical and molecular toxicology. 1999; 13(3‐4): 127-134

Eidi, A, Eidi, M, & Esmaeili, E. Antidiabetic effect of garlic (Allium sativum L.) in normal and streptozotocin-induced diabetic rats. Phytomedicine. 2006; 13(9-10): 624-629

Gardner, CD, Chatterjee, LM, & Carlson, JJ. The effect of a garlic preparation on plasma lipid levels in moderately hypercholesterolemic adults. Atherosclerosis. 2001; 154(1): 213-220

Ha, AW, Ying, T, & Kim, WK. The effects of black garlic (Allium satvium) extracts on lipid metabolism in rats fed a high fat diet. Nutrition research and practice. 2015; 9(1): 30-36

Jang, E-K, Seo, J-H, & Lee, S-P. Physiological activity and antioxidative effects of aged black garlic (Allium sativum L.) extract. Korean Journal of Food Science and Technology. 2008; 40(4): 443-448

Jo, H, & Choi, M. Effect of 1% garlic powder on serum and liver lipid and plasma amino acid concentration in rats fed cholesterol diet. JOURNAL-KOREAN SOCIETY OF FOOD SCIENCE AND NUTRITION. 2002; 31(1): 98-103

Jung, Y-M, Lee, S-H, Lee, D-S, You, M-J, Chung, IK, Cheon, WH, et al. Fermented garlic protects diabetic, obese mice when fed a high-fat diet by antioxidant effects. Nutrition research. 2011; 31(5): 387-396
Kwon, M-J, Song, Y-S, Choi, M-S, Park, S-J, Jeong, K-S, & Song, Y-O. Cholesteryl ester transfer protein activity and atherogenic parameters in rabbits supplemented with cholesterol and garlic powder. Life sciences. 2003; 72(26): 2953-2964

Larsson, SC. Dietary fats and other nutrients on stroke. Current opinion in lipidology. 2013; 24(1): 41-48
Lee, Y-M, Gweon, O-C, Seo, Y-J, Im, J, Kang, M-J, Kim, M-J, et al. Antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes mellitus. Nutrition research and practice. 2009; 3(2): 156-161

Lin, MC, Wang, E-J, Lee, C, Chin, K, Liu, D, Chiu, J-F, et al. Garlic inhibits microsomal triglyceride transfer protein gene expression in human liver and intestinal cell lines and in rat intestine. The Journal of nutrition. 2002; 132(6): 1165-1168

Rahman, K, & Lowe, GM. Garlic and cardiovascular disease: a critical review. The Journal of nutrition. 2006; 136(3): 736S-740S

Ried, K, Toben, C, & Fakler, P. Effect of garlic on serum lipids: an updated meta-analysis. Nutrition reviews. 2013; 71(5): 282-299

Seo, Y-J, Gweon, O-C, Im, J, Lee, Y-M, Kang, M-J, & Kim, J-I. Effect of garlic and aged black garlic on hyperglycemia and dyslipidemia in animal model of type 2 diabetes mellitus. J Food Sci Nutr. 2009; 14(1): 1-7

Shin, JH, Lee, CW, Oh, SJ, Yun, J, Kang, MR, Han, S-B, et al. Hepatoprotective effect of aged black garlic extract in rodents. Toxicological research. 2014; 30(1): 49

Sushama,M. ; Eswarudu, M.M. ; Venkateshwaralu, G and Radhika,P.(2012) Evaluation of anti epileptic activity of Pisidium Guajana leaves extract in mise I nt.J of research of pharma and Biom.Sci, 3(2): 887-886

Yeh, Y-Y, & Liu, L. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: human and animal studies. The journal of nutrition. 2001; 131(3): 989S-993S
Published
2020-06-01
How to Cite
UoD, J. of D. U., HAJI, A. R., ABDAL, T. A., ALI, B. A., & AHMED, M. S. (2020). EFFECT OF GARLIC OIL ON SOME BIOCHEMICAL PARAMETERS IN RATS EXPOSED TO HYDROGEN PEROXIDE. Journal of Duhok University, 22(2), 189-195. https://doi.org/10.26682/ajuod.2019.22.2.18
Section
Agriculture and Veterinary Science

Most read articles by the same author(s)

<< < 1 2