MOLECULAR IDENTIFICATION OF CUCURBIT FLY Dacus ciliatus (DIPTERA: TEPHRITIDAE) INFEST CUCURBITACEAE FAMILY BASED ON MITOCHONDRIAL GENE IN KURDISTAN REGION- IRAQ
Fruit fly hosts survey was carried out in many villages at three provinces (Duhok, Erbil and Sulaimaniyah /Kurdistan region, Iraq) during period 15/9-1/12/2017 and 15/5-30/9/2018. Ethiopian fruit fly Dacus ciliates (Loew) which belongs to the genus Dacus, family Tephritidae, order Diptera were found infesting most vegetables like cucubitaceae and some of fruit trees like fig. For molecular identification, polymerase chain reaction (PCR) amplification technique was used to amplify a single or a few copies of a pieces of DNA to millions of copies of a particular DNA sequence. For rearing this pest, damaged fruits were collected and kept in a round cages galvanized by sieves cloth (35 cm diameter, 40 cm hight), containing a layer of 3 cm soil to facilitate pupation
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