MOLECULAR DETECTION OF ORAL TRICHOMONAS TENAX AMONG INDIVIDUALS ATTENDING DENTAL CARE UNITS USING PCR IN DUHOK CITY – KURDISTAN REGION

  • NADIA TAWFIQ JAFFER Dept.of prosthetic and conservative dentistry, college of dentistry, University of Duhok, Kurdistan Region-Iraq
  • ALEEN SARDAR AL-NOORI Dept. of microbiology, college of medicine, University of Duhok, Kurdistan Region-Iraq
  • AHMED MOHAMMED SALIH Duhok Medical Research Centre (DMRC), college of medicine, University of Duhok, Kurdistan Region-Iraq
  • SOLEEN SARDAR ZUHDI Duhok Medical Research Centre (DMRC), college of medicine, University of Duhok, Kurdistan Region-Iraq
Keywords: KEY WORDS: Trichomonas tenax, oral cavity status, gingivitis, PCR

Abstract

ABSTRACT          

Background

Trichomonas tenax is a flagellated protozoan which inhabits theoral cavity of human and usually found in persons with poor oral hygiene and periodontitis. This is the first study in Duhok and Kurdistan region that aims at the detection of T. tenax in patients with oral diseases based on conventional PCR.

Subjects and Methods

In this cross sectional study, a total of 184 individuals with different mouth conditions were included from October 2017 to February 2018 who attended the dental complex and outpatients dentistry clinic at the college of dentistry – Duhok University. Oral swab was taken from each subject for  DNA extraction. The  extracted DNA was subjected to PCR in order to detect T. tenax , using the 18s rRNA gene specific primers.

Results

Out of the 184 subjects involved in the study, 94(51.09%) were males and 90(48.91%) were females and the mean age was 48+5.2 years. Regarding the oral condition on examination, 40.24% have had healthy gingival and 8.67% of them have had gingivitis. Only 8(4.35%) of the total subjects were positive for T. tenax and all of them were among the female group while all of the males involved in the study were negative for T. tenax. Statistically the difference between males and females was significant(p=0.04) regarding the existence of oral T. tenax. The highest rate of T. tenax presence was found in the age group 20-30 years with healthy gingival (1.62%) followed by age group 51years and above with gingivitis and these differences were significant(p=0.03), and then the age group 31-40 years (0.54%) both with healthy gingival and gingivitis. Regarding the dentation condition of the participants, T. tenax was detected in complete dentate (1.08%), partial dentate without prosthesis (2.16%) with a significant differenc(p=0.03), partial edentulous with removable prosthesis (0.54%) and partial edentulous with wearing denture (0.54%).

Conclusion

Trichomonas tenax was detected in female group only and all males were negative. The dentation status had a significant effect on the presence of oral T. tenax in positive females.

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References

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Published
2020-06-05
How to Cite
JAFFER, N. T., AL-NOORI, A. S., SALIH, A. M., & ZUHDI, S. S. (2020). MOLECULAR DETECTION OF ORAL TRICHOMONAS TENAX AMONG INDIVIDUALS ATTENDING DENTAL CARE UNITS USING PCR IN DUHOK CITY – KURDISTAN REGION. Journal of Duhok University, 22(2), 195-202. https://doi.org/10.26682/sjuod.2019.22.2.22
Section
Pure and Engineering Sciences