EFFICACY OF PLATELET RICH FIBRIN PREPARED WITH TITANIUM TUBES AND PLATELET RICH FIBRIN PREPARED WITH PLASTIC TUBES IN SOFT TISSUE WOUND HEALING:  AN EXPERIMENTAL STUDY IN SHEEP

JIYAR M.  NAJI; OMAR  HAMEED

doi:10.26682/sjuod.2019.22.2.23

JIYAR M. NAJI Dept. of Oral Surgery, College of dentistry, University of Duhok,
OMAR HAMEED Dept. of Oral Surgery, College of dentistry, University of Duhok,

DOI: https://doi.org/10.26682/sjuod.2019.22.2.23

Keywords: KEYWORDS: titanium tubes, platelet rich fibrin, plastic tubes.

Abstract

ABSTRACT

We have used a developed new titanium-prepared, platelet-rich fibrin (T-PRF) with the protocol for making it, which is established on the hypothesis that titanium tubes may be more effective at activating platelets than the glass tubes used by Chouckroun in his platelet-rich fibrin (PRF) method (tunali et al 2013). The aim of this study was to investigate the efficacy of T-PRF and compare with L-PRF for soft tissue wound healing. Blood samples from 10 sheep were used to confirm the protocol for formation of T-PRF and L-PRF to develop the experiment. The mucoperiosteal flaps were filled with autologous T-PRF membranes and L-PRF in the mandible and maxillary regions.

Samples collected from the surgical sites were stained with haematoxylin and eosin. We found that after 14 days of membranes implantation there was no significant differences between there histological sections. While in histological section of 28 days, the PRF prepared with titanium tube has effect in soft tissue with much and more organized collagen and less inflammatory cells reaction in compare with PRF prepared with plastic tubes effect in soft tissue wound healing. Both tubes had been centrifuged for 15 min at 3500 rpm.

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References

REFERENCES
Mustafa tunali, Hakan ozdemir et al. A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin. http://dx.doi.org/10.1155/2014/209548 .
Anitua E, Sánchez M, Nurden AT, Nurden P, Orive G, Andia I. New insights into and novel applications for platelet-rich fibrin therapies. Trends Biotechnol 2006;24:227–34.
Choukroun J, Adda F, Schoeffler C, Vervelle A. An opportunity in perioimplantology: the PRF. Implantodontie 2001;42:55–62 [in French].
Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e37–44.
Dohan DM, Choukroun J, Diss A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates? Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e51–5.
Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 2009;27:158–67.
Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e56–60.
Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:299–303.
Del Corso M, Sammartino G, Dohan Ehrenfest DM. Re: Clinical evaluation of a modified coronally advanced flap alone or in combination with a platelet-rich fibrin membrane for the treatment of adjacent multiple gingival recessions: a 6-month study. J Periodontol 2009;80:1694–7.
Huang FM, Yang SF, Zhao JH, Chang YC. Platelet-rich fibrin increases proliferation and differentiation of human dental pulp cells. J Endod 2010;36:1628–32.
Sharma A, Pradeep AR. Autologous platelet rich fibrin in the treatment of mandibular degree II furcation defects: a randomized clinical trial. J Periodontol 2011;82:1396–403.
Simonpieri A, Choukroun J, Del Corso M, Sammartino G, Dohan Ehrenfest DM. Simultaneous sinus-lift and implantation using microthreaded implants and leukocyte- and platelet-rich fibrin as sole grafting material: a six-year experience. Implant Dent 2011;20:2–12.
O’Connell SM, Impeduglia T, Hessler K, Wang XJ, Carroll RJ, Dardik H. Autologous platelet-rich fibrin matrix as cell therapy in the healing of chronic lower-extremity ulcers. Wound Repair Regen 2008;16:749–56.
Braccini F, Tardivet L, Dohan Ehrenfest DM. The relevance of Choukroun’s Platelet-Rich Fibrin (PRF) during middle ear surgery: preliminary results. Rev Laryngol Otol Rhinol (Bord) 2009;130:175–80 [in French].
Baeyens W, Glineur R, Evrard L. The use of platelet concentrates: platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) in bone reconstruction prior to dental implant surgery. Rev Med Brux 2010;31:521–7 [in French].
Inchingolo F, Tatullo M, Marrelli M. Trial with Platelet-Rich Fibrin and Bio-Oss used as grafting materials in the treatment of the severe maxillar bone atrophy: clinical and radiological evaluations. Eur Rev Med Pharmacol Sci 2010;14:1075–84.
Toffler M, Toscano N, Holtzclaw D. Osteotome-mediated sinus floor elevation using only platelet-rich fibrin: an early report on 110 patients. Implant Dent 2010;19:447–56.
Gürbüzer B, Pikdöken L, Tunali M, et al. Scintigraphic evaluation of osteoblastic activity in extraction sockets treated with platelet-rich fibrin. J Oral Maxillofac Surg 2010;68:980–9.
O’Connell SM. Safety issues associated with platelet-rich fibrin method. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007;103:587–93.
Dohan EhrenfestDM,Diss A, Odin G, Doglioli P, HippolyteMP, Charrier JB. In vitro effects of Choukroun’s PRF (platelet-rich fibrin) on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures. Oral SurgOral Med Oral Pathol Oral Radiol Endod 2009;108:341–52.
Lee E-H, Kim J-Y, Kweon HY, et al. A combination graft of lowmolecular- weight silk fibroin with Choukroun platelet-rich fibrin for rabbit calvarial defect. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;109:e33–8.
Dohan Ehrenfest DM, Del Corso M, Inchingolo F, Charrier JB. Selecting a relevant in vitro cell model for testing and comparing the effects of a Choukroun’s platelet-rich fibrin (PRF) membrane and a platelet-rich plasma (PRP) gel: tricks and traps. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010;110:409–11.
He L, Lin Y, Hu X, Zhang Y,Wu H. A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009;108:707–13.
Kinlough-Rathbone RL, Rand M, Packham MA. Rabbit and rat platelets do not respond to thrombin receptor peptides that activate human platelets. Blood 1993;82:103–6.