SPECIFIC GENES AFFECTING BODY WEIGHT IN IRAQI AWASSI SHEEP

  • YOUSIF M. S. AL-BARZINJI Dept.of Animal Resources, College of Agricultural Engineering Sciences,University of Salahaddin, Kurdistan Region-Iraq
  • ASO A. AMEEN Ministry of Agriculture &Water Resources-Kurdistan Region-Iraq
Keywords: RFLP-PCR, MSTN, CAST, MHC-DRB1, Weaning weight

Abstract

The present study was to investigate genotypes of three genes (MSTN, CAST and MHC-DRB1) that related to body weight in Awassi lambs. A total of 52 lambs were used. The DNA concentration and DNA purity (A260/280) was calculated automatically by nanodrop and ranged between 10.53-53.94 ng/μl and 1.51-2.19, respectively. The RFLP-PCR results showed that the CAST and MHC-DRB1genes were polymorphism among individuals while the MSTN gene was monomorphism. Lambs with CC genotype for MHC-DRB1 locus have positive effect on body weight at most ages while lambs with AB genotype for CAST locus gave higher body weight at all ages and higher average daily gain. The lambs with BBCCBB genotypes for the three genes under study gave higher weight at weaning (22.15 kg/lamb) and higher average daily gain (0.197 kg/lamb/day) compared with all other genotypes. These results showed that Awassi lambs have potential for growth traits and the selection process with the aid of molecular technique can play a positive and rapid role for improvement this breed of sheep.

Downloads

Download data is not yet available.

References

Al-Azzawi, W. A. R., Al-Salman, M. H., and Hamad, S.A. (1995).Relationship between body weight and dimention traits in Awassi sheep. Proceeding the Regional Symposiom on integrated Croplivestock system in west Asia and North Africa 6-8 November, Amman, Jordan; 284-290.
AL-Barzinji, Y.M.S., and Gardi, H.E.A. (2012). Prediction of breeding value of awassi rams based on progeny performance in Kurdistan region of Iraq. Proceedings of the 15th AAAP Anim. Sci. Congress; 26-30.
AL-Barzinji, Y.M.S., and Gardi, H.E.A. (2015). Genetic evaluation and factors affecting post weaning growth performance and body dimensions of Awassi lambs. J. of Zankoi Sulaimani;17 (3) (Part-A). P. 25-31.
Al-Rawi, A. A, Al-Haboby A.H., and Salman A. (1996).Small ruminants breeding, reproduction physiology research as technology transfer in Iraq (W. Mourani, N. Haddad, Eds.), ICARDA West Asia Regional Program, Amman, Jordan.
Ammer, H., Schwaiger, F.W., Kammerbauer, C., Gomolka, M., Arriens, A., Lazary, S., and Epplenj, T.(1992). Exonic polymorphism vs. intronic simple repeat hypervariability in MHC-DRB genes. Immunogenetics; 35:.332-340.
Ashrafi, F., Mardani, K., Ali, H.A., Darvishzadeh, R., and Farhadian, M. (2014). Association of molecular polymorphism in exon 2 of Ovar-DRB1 gene with weight traits in Iranian Makuie sheep breed. Turk J Vet Anim., Sci. ; 38:126-132.
Baumung, R., Simianer, H.H., and Hoffmann, I. (2004). Genetic diversity studies in farm animals - a survey. J Anim Breed Genet; 121. P. 361–373.
Chu, M. X., Liu, Z.H., Jiao, C.L., He, Y.Q., Fang, L., Ye, S.C., Chen, G.H., and Wang, J,Y.(2007). Mutation in BMPRIB and BMP15 genes are association with litter size in Small Tailed Han sheep (Ovis aries). J Anim Sci; 85:.598-603.
Davis. G.H., Galloway, S.M., Ross, I.K., Gregen, S.M., Ward, J., Nimbkar, B.V., Ghalsasi, P.M., Nimbkar, C., et al. (2000). DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. Biol Reprod ; 66:1869 1874.
FAO, (Food and Agriculture Organization), (2000). Quarterly bulletin of statistics 1. FAO. Rome, Italy.
Gabor, M.A., Trakovicha, A., and Miluchova, M. (2009). Analysis of polymorphism of CAST gene and Clpg gene in sheep by PCR-RFLP method. J. Zoot. Biotehnol; 42: 470-476.
Gorlov, I., Shirokova, V., Randelin, A., Voronkova, V., Mosolova, N., Zlobina, E., etal. (2016). CAST/MspI gene polymorphism and its impact on growth traits of Soviet Merino and Salsk sheep breeds in the South European part of Russia. Turk J Vet Anim Sci.; 40:399-405.
Hanrahan, J. P., Gregen, S. M., Mulsant, P., Mullen, M., Davis G. H., Powell R. and Galloways, S.(2004). Mutations in the genes for oocyte derived growth factors GDF9 and BMP15 are associated sheep (Ovis aries). Biol. Reprod. 70. P.900-909.
Khan, S., Riaz, M., Ghaffar, A., and Khan, M. (2012). Calpastatin (CASTA) gene polymorphism and its association with average daily weight gain in Balkhi and Kajli sheep and Beetal Goat breeds. Pakistan J Zool ; 44(2): 377-382.
Moradband, F., Rahimi, G., and Gholizadeh, M.(2011). Association of polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep. Asian-Aust J Anim Sci ; 24 (9): 1179-1183.
Nanekarani, S., Asadi, N., Khederzadeh, S.(2001). Genotypic frequency of calpastatin gene in lori sheep by PCR-RFLP method . 2nd International Conference on Environmental Science and Development IPCBEE vol.4, IACSIT Press, Singapore.
SAS, Statistical Analyses System.(2002). SAS/STAT User’s Guide, Version 8, first edition, Cary, NC, USA.
Shahram, N. and Majid, G. (2014). Polymorphism of candidate genes for meat production in Lori sheep. IERI Procedia; 8: 18 – 23.
Shahroudi, F. E., Nassiry, M. R., Valizadh, R., Moussavi, A.H., Tahmoorespour, M., and Ghiasi, H. (2006). Genetic polymorphism at MTNR1A, CAST and CAPN loci in Iranian Karakul sheep. Iranian J Biotechnology; 4(2): 117-122.
Williams, J.L. (2005). The use of marker-assisted selection in animal breeding and ‎biotechnology. Scientific and Technical Review, Office International Des Epizooties ; 24:379-391.
Xianglong, L., Zhengzhu, I., Rongyan, L., Guiru, Z., Yuanfang, Z. G., and Lanhui, L. I. (2008). Deletion of TTTTA in 5´UTR of goat MSTN gene and its distribution and genetic effect on body weight. Front. Agric. China; 2(1): 103-109.
Published
2020-07-12
How to Cite
AL-BARZINJI, Y. M. S., & AMEEN, A. A. (2020). SPECIFIC GENES AFFECTING BODY WEIGHT IN IRAQI AWASSI SHEEP . Journal of Duhok University, 22(s2), 187-195. https://doi.org/10.26682/cajuod.2020.22.2.21