THE RELATIONSHIP OF GLIOMAS WITH TP53 RS1042522 C > G AND GLIOMAS IN DUHOK
Keywords:
PCR- RFLP; TP53; Codon72; SNPs; Brain Cancer (Gliomas).
Abstract
A tumor suppressor gene TP53 has a central role in controlling the cell cycle, apoptosis, as well as
DNA damage repair. A common polymorphism in TP53 is the Arg72Pro exon 4 polymorphism.
Polymorphism has been proposed to be associated with genetically determined susceptibility in different
types of cancers, including glioma. This study was conducted to estimate the distribution of glioma within
age groups, gender, smokers, and residence of individual also to investigate the distribution of TP53
Arg72Pro SNPs genotype in glioma, and determine whether TP53 Arg72Pro polymorphism is a possible
relevance in susceptibility to glioma using RFLP-PCR analysis. Enrolled were 65 patients (glioma tissues
matched age and gender) and 70 healthy individuals as a control. The findings in glioma samples
40(61.54%) were homozygous for arginine (Arg / Arg), 19 (29.23%) heterozygous for (Arg / Pro), and 6
(9.23%) homozygous for proline (Pro / Pro). Three separate frequencies of genotypes of Arg72Pro; 33
(47.14%), 28 (40.0), and 9 (12.86%) were identified in healthy individuals, respectively. The allele
Frequencies for the Pro 72 and Arg 72 gliomas were 16 (24.62%) and 49 (75.38%), respectively. In the Pro
72 and Arg 72 controls, the allele frequencies were 23 (32.86%) and 47 (67.14%), respectively. Finally,
there was no significant relationship between age group, gender, dwellers, non-smokers and smokers in
different genotypes of codon 72 of TP53 gene (P < 0.05).
Downloads
Download data is not yet available.
References
Mamelak, A.N., and Jacoby, D.B., (2007). "Targeted
delivery of antitumoral therapy to glioma and
other malignancies with synthetic chlorotoxin
(TM-601)". Expert Opinion on Drug
Delivery. 4 (2), 175-186.
Sarkar, C., Jain, A., and Suri, V., (2009). Current
concept in the pathology and genetics of
glioma. Indian journal of cancer. 49(2), 108-
119.
Prerana, J.h.a., Pankaj, J.h.a., Pankaj, P., Kunzang,
C., Vaishali, S., Mehar, C.S., Guresh
K., Manmohan, S., Ashok Kumar, M., Chitra
S., (2011). TP53 polymorphisms in gliomas
from Indian patients: Study of codon 72
genotype, rs1642785, rs1800370 and 16 base
pair insertion in intron-3. Exp Mol Pathol.
90(2), 167-72. Bondy, M.L., Scheurer, M.E., Malmer, B., Barnholtz-
Sloan, J.S., Davis, F.G., Il'yasova, D., Brain
Tumor Epidemiology, C. (2008). Brain tumor
epidemiology: consensus from the Brain
Tumor Epidemiology Consortium. Cancer.
113(7 Suppl), 1953-1968.
Baldwin, R.T., and Preston-Martin S., (2004).
Epidemiology of brain tumors in childhood--a
review. Toxicol Appl Pharmacol. 199(2), 118-
31.
Sadetzki, S., Flint-Richter, P., Starinsky, S., Novikov,
I., Lerman, Y., Goldman, B., et al (2005).
Genotyping of patients with sporadic and
radiation-associated meningiomas. Cancer
Epidemiol Biomarkers Prev. 14, 969–76.
Umansky, F., Shoshan, Y., Rosenthal, G., Fraifeld,
S., Spektor, S., (2008). Radiation-Induced
Meningioma. Neurosurg Focus. 24 (5), E7.
Inskip, P.D., Hoover, R.N., Devesa, S.S., (2010).
Brain cancer incidence trends in relation to
cellular telephone use in the United States,
Neuro Oncol. 1211, 1147-1151.
Cardis, E., and Sadetzki, S., (2011). Indications of
possible brain-tumour risk in mobile-phone
studies: should we be concerned? Occup
Environ Med.
Chien, L.N., Gittleman, H., Ostrom, Q.T., Hung,
K.S., Sloan, A.E., Hsieh, Y.C., Barnholtz-
Sloan, J.S., (2016). Comparative Brain and
Central Nervous System Tumor Incidence and
Survival between the United States and
Taiwan Based on Population-Based Registry.
Front Public Health, 4, 151.
Scarbrough, P.M., Akushevich, I.., Wrensch, M., &
Il'yasova, D., (2014). Exploring the
association between melanoma and glioma
risks. Ann Epidemiol, 24(6), 469-474.
Khan, A.A., Pandith, A.A., Wani, A.A., Malik, N.K.,
et al (2015). Significant Association of Proline
Allele in TP53 Gene, Arg72pro Polymorphism
in Brain Tumors and its Prognostic Value.
Journal of Cancer Science & Therapy Journal
of Cancer Science & Therapy. 7(7), 236-241.
Lannering, B., Sandstrom, P.E., Holm, S., et al
(2009). Classification, incidence and survival
analyses of children with CNS tumours
diagnosed in Sweden 1984-2005. Acta
Paediatr. 98(10), 1620-7.
Dolecek, T.A., Propp, J.M., Stroup, N.E., Kruchko
C., (2012). CBTRUS statistical report: primary
brain and central nervous system tumors
diagnosed in the United States in 2005- 2009.
Neuro-oncology; 14 Suppl 5, v1-49.
Kruiswijk, F., Labuschagne, C.F., Vousden, K.H.,
(2015). P53 in survival, death and metabolic
health: a lifeguard with a licence to kill. Nat
Rev Mol Cell Biol. 16, 393-405.
Yang, Z., Nie, S., Zhu, H., Wu, X., Jia, S., Luo, Y.,
Tang, W., (2013). Association of p53
Arg72Pro polymorphism with bladder cancer:
a meta-analysis. Gene; 512(2), 408–13.
Dastjerdi, M.N., (2011). TP53 codon 72
polymorphism and P53 protein expression in
colorectal cancer specimens in Isfahan. Acta
Med Iran. 49, 71–77.
Hu, X., Zhang, Z., Ma, D., Huettner, P.C., Massad,
L.S., Nguyen, L., Borecki, I., and Rader, J.S.,
(2010). TP53, MDM2, NQO1 and
susceptibility to cervical cancer. Cancer
Epidemiol Biomarkers Prev. 19, 755–761.
Sharma, S., Sambyal, V., Guleria, K., Manjari, M., et
al (2014). TP53 Polymorphisms in Sporadic
North Indian Breast Cancer Patients. Asian
Pac J Cancer Prev. 15 (16), 6871-6879.
Zhuo, X.L., Cai, L., Xiang, Z.L., Zhuo, W.L., Wang,
Y., Zhang, X.Y., (2009). TP53 codon 72
polymorphism contributes to nasopharyngeal
cancer susceptibility: a meta-analysis. Arch
Med Res. 40, 299-305.
Rogler, A., Rogenhofer, M., Borchardt, A., Lunz,
J.C., Knoell, A., Hofstaedter, F., et al (2011).
P53 codon 72 (Arg72Pro) polymorphism and
prostate cancer risk: association between
disease onset and proline genotype.
Pathobiology: journal of immunopathology,
molecular and cellular biology. 78, 193-200.
Lin, H.Y., Huang, C.H., Yu, T.J., Wu, W.J., Yang,
M.C., Lung, F.W., (2012). P53 codon 72
polymorphism as a progression index for
bladder cancer. Oncology reports. 27, 1193-9.
Qiao, Q., and Hu, W., (2013). The association
between TP53 Arg72Pro polymorphism and
lung cancer susceptibility: evidence from
30,038 subjects. Lung. 191, 369-77.
Rebbani, K., Marchio, A., Ezzikouri, S., Afifi, R.,
Kandil, M., Bahri, O., et al (2015). TP53
R72P polymorphism modulates DNA
methylation in hepatocellular carcinoma. Mol
Cancer. 14, 74.
Hossain, A., Murshid G.M.M., Zilani, M.N.H., Islam,
F., Sultana, R., et al (2017). TP53 codon 72
polymorphism and breast cancer risk in
Bangladeshi population. Breast Cancer 24,
571–578.
Liu P, Zhuo Z, Li W, Cheng J, Zhou H, et al (2019).
TP53 rs1042522 C>G polymorphism and
Wilms tumor susceptibility in Chinese
children: a four-center case-control study.
Biosci. Rep. 39, BSR20181891.
Bass, F, Bikker, H, Ommen, GJ and Vijlder, J (1984).
Unusual scarcity of restriction site
polymorphisms in human thyroglobin gene: A
linkage study suggesting autosomal
dominance of defective thyroglobin
allele.Human genetics. 67, 301-305. Maniatis, T., Fritch, E.F., and Sambrook, J., (1982).
Molecular cloning: A laboratory manual, Cold
Spring Harbor Laboratory Press, New York.
Pinto, G.R., Yoshioka, F.K., Silva, R.L., Clara, C.A.,
et al (2008). Prognostic value of TP53
Pro47Ser and Arg72Pro single nucleotide
polymorphisms and the susceptibility to
gliomas in individuals from Southeast Brazil.
Genet. Mol. Res. 7, 207-216.
Kirk, B.W., Feinsod, M., Favis, R., Kliman, R.M., et
al (2002). Single nucleotide polymorphism
seeking long term association with complex
disease. Nucleic Acids Res. 30, 3295-3311.
-Peris-Bonet, R., Martinez-Garcia, C., Lacour, B., et
al (2006). Childhood central nervous system
tumours--incidence and survival in Europe
(1978-1997): report from Automated
Childhood Cancer Information System project.
Eur J Cancer; 42(13), 2064-80.
Aydin, D., Feychting, M., Schüz, J., et al (2011).
Impact of random and systematic recall errors
and selection bias in case-control studies on
mobile phone use and brain tumors in
adolescents (CEFALO study).
Bioelectromagnetics, 32, 396-407.
Hamajima, N., Matsuo, K., Suzuki, T., Nakamura, T.,
Matsuura, A., Hatooka, S., Shinoda, M.,
Kodera, Y., Yamamura, Y., Hirai, T., Kato, T.,
Tajima, K., (2002). No associations of p73
G4C14-to-A4T14 at exon 2 and p53 Arg72Pro
polymorphisms with the risk of digestive tract
cancers in Japanese. Canc. Lett., 181(1), 81-
85.
Khadang, B., Fattahi, M.J., Talei, A., Dehaghani,
A.S., Ghaderi, A., (2007). Polymorphism of
TP53 codon 72 showed no association with
breast cancer in Iranian women. Canc. Genet.
Cytogenet. 173, 38-42.
Onrat, S.T., Ellidokuz, E., Kupelioglu, A., Durhan,
E., (2009). Frequency of TP53 codon72
polymorphism in cases with colon cancer.
Turk. J. Canc., 39(1), 5-10.
Moradinasab, M., Ostovar, A., Nabipour, I., Eghbali,
S.S., Vahdat, K., Ghaderi, A., Mohamad Reza
Farzaneh, M.R., Ravanbod, M.R., (2017).
TP53 Codon 72 Genetic Polymorphism,
rs1042522, Modifies the Association Between
Tobacco Smoking and Breast Cancer Risk.Iran
Red Crescent Med J. 19(10), e58027.
Fang, Y., Wu, X., Lin Li, Zhu, J., Wu,
H., Zhou, H., He, J., and Wang, Y., (2020).
TP53 Arg72Pro polymorphism and
neuroblastoma susceptibility in eastern
Chinese children: a three-center case–control
study. Biosci Rep. 40(5), BSR20200854.
Mammano, E., Belluco, C., Bonafe, M., Olivieri, F.,
Mugianesi, E., Barbi, C., Mishto, M., Cosci,
M., Franceschi, C., Lise, M., Nitti, D., (2009).
Association of p53 polymorphisms and
colorectal cancer: Modulation of risk and
progression. EJSO. 35(4), 415-419.
Zhu, Z.Z., Wang, A.Z., Jia, H.R., Jin, X.X., He, X.L.,
Hou, L.F., Zhu, G., (2007). Association of the
TP53 Codon 72 Polymorphism with colorectal
cancer in a Chinese population. Jpn. J. Clin.
Oncol. 37(5), 385-390.
Doosti, A., Zamani, M., Dehkordi, P.G., Taheri, S.,
Banitalebi, M., and Mahmoudzadeh, M.,
(2011). Association of the p53 codon 72
polymorphism with colorectal cancer in South
West of Iran. Scientific Research and Essays
Vol. 6(15), 3148-3152.
delivery of antitumoral therapy to glioma and
other malignancies with synthetic chlorotoxin
(TM-601)". Expert Opinion on Drug
Delivery. 4 (2), 175-186.
Sarkar, C., Jain, A., and Suri, V., (2009). Current
concept in the pathology and genetics of
glioma. Indian journal of cancer. 49(2), 108-
119.
Prerana, J.h.a., Pankaj, J.h.a., Pankaj, P., Kunzang,
C., Vaishali, S., Mehar, C.S., Guresh
K., Manmohan, S., Ashok Kumar, M., Chitra
S., (2011). TP53 polymorphisms in gliomas
from Indian patients: Study of codon 72
genotype, rs1642785, rs1800370 and 16 base
pair insertion in intron-3. Exp Mol Pathol.
90(2), 167-72. Bondy, M.L., Scheurer, M.E., Malmer, B., Barnholtz-
Sloan, J.S., Davis, F.G., Il'yasova, D., Brain
Tumor Epidemiology, C. (2008). Brain tumor
epidemiology: consensus from the Brain
Tumor Epidemiology Consortium. Cancer.
113(7 Suppl), 1953-1968.
Baldwin, R.T., and Preston-Martin S., (2004).
Epidemiology of brain tumors in childhood--a
review. Toxicol Appl Pharmacol. 199(2), 118-
31.
Sadetzki, S., Flint-Richter, P., Starinsky, S., Novikov,
I., Lerman, Y., Goldman, B., et al (2005).
Genotyping of patients with sporadic and
radiation-associated meningiomas. Cancer
Epidemiol Biomarkers Prev. 14, 969–76.
Umansky, F., Shoshan, Y., Rosenthal, G., Fraifeld,
S., Spektor, S., (2008). Radiation-Induced
Meningioma. Neurosurg Focus. 24 (5), E7.
Inskip, P.D., Hoover, R.N., Devesa, S.S., (2010).
Brain cancer incidence trends in relation to
cellular telephone use in the United States,
Neuro Oncol. 1211, 1147-1151.
Cardis, E., and Sadetzki, S., (2011). Indications of
possible brain-tumour risk in mobile-phone
studies: should we be concerned? Occup
Environ Med.
Chien, L.N., Gittleman, H., Ostrom, Q.T., Hung,
K.S., Sloan, A.E., Hsieh, Y.C., Barnholtz-
Sloan, J.S., (2016). Comparative Brain and
Central Nervous System Tumor Incidence and
Survival between the United States and
Taiwan Based on Population-Based Registry.
Front Public Health, 4, 151.
Scarbrough, P.M., Akushevich, I.., Wrensch, M., &
Il'yasova, D., (2014). Exploring the
association between melanoma and glioma
risks. Ann Epidemiol, 24(6), 469-474.
Khan, A.A., Pandith, A.A., Wani, A.A., Malik, N.K.,
et al (2015). Significant Association of Proline
Allele in TP53 Gene, Arg72pro Polymorphism
in Brain Tumors and its Prognostic Value.
Journal of Cancer Science & Therapy Journal
of Cancer Science & Therapy. 7(7), 236-241.
Lannering, B., Sandstrom, P.E., Holm, S., et al
(2009). Classification, incidence and survival
analyses of children with CNS tumours
diagnosed in Sweden 1984-2005. Acta
Paediatr. 98(10), 1620-7.
Dolecek, T.A., Propp, J.M., Stroup, N.E., Kruchko
C., (2012). CBTRUS statistical report: primary
brain and central nervous system tumors
diagnosed in the United States in 2005- 2009.
Neuro-oncology; 14 Suppl 5, v1-49.
Kruiswijk, F., Labuschagne, C.F., Vousden, K.H.,
(2015). P53 in survival, death and metabolic
health: a lifeguard with a licence to kill. Nat
Rev Mol Cell Biol. 16, 393-405.
Yang, Z., Nie, S., Zhu, H., Wu, X., Jia, S., Luo, Y.,
Tang, W., (2013). Association of p53
Arg72Pro polymorphism with bladder cancer:
a meta-analysis. Gene; 512(2), 408–13.
Dastjerdi, M.N., (2011). TP53 codon 72
polymorphism and P53 protein expression in
colorectal cancer specimens in Isfahan. Acta
Med Iran. 49, 71–77.
Hu, X., Zhang, Z., Ma, D., Huettner, P.C., Massad,
L.S., Nguyen, L., Borecki, I., and Rader, J.S.,
(2010). TP53, MDM2, NQO1 and
susceptibility to cervical cancer. Cancer
Epidemiol Biomarkers Prev. 19, 755–761.
Sharma, S., Sambyal, V., Guleria, K., Manjari, M., et
al (2014). TP53 Polymorphisms in Sporadic
North Indian Breast Cancer Patients. Asian
Pac J Cancer Prev. 15 (16), 6871-6879.
Zhuo, X.L., Cai, L., Xiang, Z.L., Zhuo, W.L., Wang,
Y., Zhang, X.Y., (2009). TP53 codon 72
polymorphism contributes to nasopharyngeal
cancer susceptibility: a meta-analysis. Arch
Med Res. 40, 299-305.
Rogler, A., Rogenhofer, M., Borchardt, A., Lunz,
J.C., Knoell, A., Hofstaedter, F., et al (2011).
P53 codon 72 (Arg72Pro) polymorphism and
prostate cancer risk: association between
disease onset and proline genotype.
Pathobiology: journal of immunopathology,
molecular and cellular biology. 78, 193-200.
Lin, H.Y., Huang, C.H., Yu, T.J., Wu, W.J., Yang,
M.C., Lung, F.W., (2012). P53 codon 72
polymorphism as a progression index for
bladder cancer. Oncology reports. 27, 1193-9.
Qiao, Q., and Hu, W., (2013). The association
between TP53 Arg72Pro polymorphism and
lung cancer susceptibility: evidence from
30,038 subjects. Lung. 191, 369-77.
Rebbani, K., Marchio, A., Ezzikouri, S., Afifi, R.,
Kandil, M., Bahri, O., et al (2015). TP53
R72P polymorphism modulates DNA
methylation in hepatocellular carcinoma. Mol
Cancer. 14, 74.
Hossain, A., Murshid G.M.M., Zilani, M.N.H., Islam,
F., Sultana, R., et al (2017). TP53 codon 72
polymorphism and breast cancer risk in
Bangladeshi population. Breast Cancer 24,
571–578.
Liu P, Zhuo Z, Li W, Cheng J, Zhou H, et al (2019).
TP53 rs1042522 C>G polymorphism and
Wilms tumor susceptibility in Chinese
children: a four-center case-control study.
Biosci. Rep. 39, BSR20181891.
Bass, F, Bikker, H, Ommen, GJ and Vijlder, J (1984).
Unusual scarcity of restriction site
polymorphisms in human thyroglobin gene: A
linkage study suggesting autosomal
dominance of defective thyroglobin
allele.Human genetics. 67, 301-305. Maniatis, T., Fritch, E.F., and Sambrook, J., (1982).
Molecular cloning: A laboratory manual, Cold
Spring Harbor Laboratory Press, New York.
Pinto, G.R., Yoshioka, F.K., Silva, R.L., Clara, C.A.,
et al (2008). Prognostic value of TP53
Pro47Ser and Arg72Pro single nucleotide
polymorphisms and the susceptibility to
gliomas in individuals from Southeast Brazil.
Genet. Mol. Res. 7, 207-216.
Kirk, B.W., Feinsod, M., Favis, R., Kliman, R.M., et
al (2002). Single nucleotide polymorphism
seeking long term association with complex
disease. Nucleic Acids Res. 30, 3295-3311.
-Peris-Bonet, R., Martinez-Garcia, C., Lacour, B., et
al (2006). Childhood central nervous system
tumours--incidence and survival in Europe
(1978-1997): report from Automated
Childhood Cancer Information System project.
Eur J Cancer; 42(13), 2064-80.
Aydin, D., Feychting, M., Schüz, J., et al (2011).
Impact of random and systematic recall errors
and selection bias in case-control studies on
mobile phone use and brain tumors in
adolescents (CEFALO study).
Bioelectromagnetics, 32, 396-407.
Hamajima, N., Matsuo, K., Suzuki, T., Nakamura, T.,
Matsuura, A., Hatooka, S., Shinoda, M.,
Kodera, Y., Yamamura, Y., Hirai, T., Kato, T.,
Tajima, K., (2002). No associations of p73
G4C14-to-A4T14 at exon 2 and p53 Arg72Pro
polymorphisms with the risk of digestive tract
cancers in Japanese. Canc. Lett., 181(1), 81-
85.
Khadang, B., Fattahi, M.J., Talei, A., Dehaghani,
A.S., Ghaderi, A., (2007). Polymorphism of
TP53 codon 72 showed no association with
breast cancer in Iranian women. Canc. Genet.
Cytogenet. 173, 38-42.
Onrat, S.T., Ellidokuz, E., Kupelioglu, A., Durhan,
E., (2009). Frequency of TP53 codon72
polymorphism in cases with colon cancer.
Turk. J. Canc., 39(1), 5-10.
Moradinasab, M., Ostovar, A., Nabipour, I., Eghbali,
S.S., Vahdat, K., Ghaderi, A., Mohamad Reza
Farzaneh, M.R., Ravanbod, M.R., (2017).
TP53 Codon 72 Genetic Polymorphism,
rs1042522, Modifies the Association Between
Tobacco Smoking and Breast Cancer Risk.Iran
Red Crescent Med J. 19(10), e58027.
Fang, Y., Wu, X., Lin Li, Zhu, J., Wu,
H., Zhou, H., He, J., and Wang, Y., (2020).
TP53 Arg72Pro polymorphism and
neuroblastoma susceptibility in eastern
Chinese children: a three-center case–control
study. Biosci Rep. 40(5), BSR20200854.
Mammano, E., Belluco, C., Bonafe, M., Olivieri, F.,
Mugianesi, E., Barbi, C., Mishto, M., Cosci,
M., Franceschi, C., Lise, M., Nitti, D., (2009).
Association of p53 polymorphisms and
colorectal cancer: Modulation of risk and
progression. EJSO. 35(4), 415-419.
Zhu, Z.Z., Wang, A.Z., Jia, H.R., Jin, X.X., He, X.L.,
Hou, L.F., Zhu, G., (2007). Association of the
TP53 Codon 72 Polymorphism with colorectal
cancer in a Chinese population. Jpn. J. Clin.
Oncol. 37(5), 385-390.
Doosti, A., Zamani, M., Dehkordi, P.G., Taheri, S.,
Banitalebi, M., and Mahmoudzadeh, M.,
(2011). Association of the p53 codon 72
polymorphism with colorectal cancer in South
West of Iran. Scientific Research and Essays
Vol. 6(15), 3148-3152.
Published
2020-12-13
How to Cite
NERWEYI, F. F. A. (2020). THE RELATIONSHIP OF GLIOMAS WITH TP53 RS1042522 C > G AND GLIOMAS IN DUHOK. Journal of Duhok University, 23(2), 157-165. https://doi.org/10.26682/sjuod.2020.23.2.17
Section
Pure and Engineering Sciences
It is the policy of the Journal of Duhok University to own the copyright of the technical contributions. It publishes and facilitates the appropriate re-utilize of the published materials by others. Photocopying is permitted with credit and referring to the source for individuals use.
Copyright © 2017. All Rights Reserved.
