MOLECULAR IDENTIFICATION AND INSECTICIDAL ACTIVITY OF Albifimbria verrucaria ISOLATED FROM CUCURBIT PLANTS AND SOIL IN IRAQ
Keywords:
Albifimbria verrucaria, Myrothecium, insecticidal activity, Epilachna chrysomelina, soil, Iraq
Abstract
Albifimbria (=Myrothecium) verrucaria (Alb. & Schwein.) L. Lombard & Crous. has been involved as
promising bio-pesticides for wide range of weeds and nematodes. Furthermore, has a promising
insecticidal activity because of its ability to produce many enzymes as lipase, protease and kinase that
have the ability to degrading the cuticles of insects. Seven isolates of A. verrucaria were isolated from
cucurbit plants and the soil. The identification was based on molecular (ITS-rDNA) and morphological
analyses. Laboratory bioassays to evaluate the insecticidal activity of A. verrucaria (cgh-38) against
different stages of squash beetle, Epilachna chrysomelina (F.) (Coleoptera: Coccinellidae) were conducted.
Three days post treatment, 87.5% mortality rate was obtained when the 1st instar larvae treated,
compared to 31.45 for 4th instar larvae. The highest mortality percent was 25 % for adults after 9 days of
treatment.
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References
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methods and applications;p.315-322.Academic
Press,New York.
Zhao YJ, Li BJ, ShiYX and Xie XW (2009).
Identification of the pathogen of tomato
Myrotheciumleaf spot(in Chinese).Acta
Phytopathological ICA Sinica 39(5), 528–531.
foliar endophytic fungi: progress, challenges,
and frontiers. Fungal Biology Reviews 21: 51–
66.
Baute MA, Deffieux G, Baute R, Neveu A (1978).
New antibiotics from the fungus Epicoccum
nigrum. I. Fermentation, isolation and
antibacterial properties. Journal of Antibiotics
31(11): 1099-1101.
Chavan S, Kolomiets E, Kuptsov V, Mandrik M,
Kulkarni M. and Deshpande MV (2009).
Significance of cuticle degrading enzymes
with special reference to lipase in biocontrol
of sugarcane woolly aphids. J. Mycol.
Plant.Pathol., 39, 118–123.
Chen Y, Ran SF, Dai DQ,Wang Y, Hyde KD,Wu
YM and JiangYL (2016). Mycosphere Essays
2. Myrothecium. Mycosphere 7(1): 64–80.
Hoagland RE, Weaver MA and Boyette CD (2007).
Myrothecium verrucaria fungus; A
Li BJ and Zhao YJ (2009). The identification of paint
spot pathogen on eggplant(in Chinese). China
Vegetable 15, 20–21.
Li Z, Chang P, Gao L and Wang X (2020). The
Endophytic Fungus Albifimbria verrucaria
from Wild Grape as an Antagonist of Botrytis
cinerea and Other Grape Pathogens.
Phytopathology 110(4): 843–850.
Liu JK, Hyde KD, Jones EBG, Ariyawansa HA, Bhat
D J, Boonmee S, et al. (2015). Fungal
diversity notes 1–110: taxonomic and
phylogenetic contributions to fungal species.
Fungal Diversity 72 (1), 1–197.
Liu WZ, Tan H, Pan QL, Li HH, Yan HJ, Tan YZ,
Zhang WM (2014). Chemical constituents of
the mycelia of an endophytic Myrothecium
roridumfrom Pogostemon cablin(Blanco)
Benth(in Chinese). Journal of Guangdong
Pharmaceutical University 30(4), 427–429.
Lombard L, Houbraken J, Decock C, Samson R A,
Meijer M, Réblová M, Groenewald J Z, Crous
P W (2016). Generic hyper-diversity in
Stachybotriaceae. Persoonia 36, 2016: 156–
246.
Mohamed, GS (2016). Pathogenicity of
entomopathogenic fungus Beauveria bassiana
and bacterium Bacillus thuringiensis var.
kurstaki against the lesser grain borer
Rhyzopertha dominica F. (Coleoptera:
Bostrichidae) under laboratory conditions. J.
Basic and Applied Mycology (Egypt) 7: 39-
44.
Nguyen LTT, Jang JY, Kim TY, Yu NH, Park AR,
Lee S, Bae Ch, Yeo JH, Hur JS, Park HW and
Kim JCh (2018). Nematicidal activity of
verrucarin A and roridin A isolated from
Myrothecium verrucaria against Meloidogyne
incognita. Pesticide Biochemistry and
Physiology 148: 133-143. Podieiablonskaia EV, Kolomytseva M P,
Myasoedova NM, Baskunov BP, Chernykh
AM, Classen T., Pietruszka J. And Golovleva
LA (2017). Myrothecium verrucaria F-3851, a
producer of laccases transforming phenolic
compounds at neutral and alkaline conditions.
Microbiology volume 86, pages370–376.
Quarles W (2011). New biopesticides for IPM and
organic production. IPM Practitioner. 33, 1-9.
Quezado Duval AM, Henz, GP, Paz-Lima ML,
Medeiros AR, Miranda BEC, Pfenning LH,
Reis A (2010). New hosts of Myrothecium
spp. in Brazil and a preliminary in vitro assay
of fungicides. Brazilian Journal of
Microbiology 41: 246-252.
Tode HJ 1790 – Fungi Mecklenburgenses Selecti.
Fasc. 1. Nova Fungorum Genera Complectens.
i-viii, 1–50, plates 1–7.
Tulloch M (1972). The genus MyrotheciumTode ex
Fr. MycologicalPapers 130, 1-42.
Vidhate R, Singh J, Ghormade V, Chavan SB, Patil A
and Deshpande MV (2015). Use of hydrolytic
enzymes of Myrothecium verrucaria and
conidia of Metarhizium anisopliae, singly and
sequentially to control pest and pathogens in
grapes and their compatibility with pesticides
used in the field. Biopestic. Int. 11(1): 48–60
(2015).
Wagenaar MM and Clardy J (2001). Two new
roridins isolated from Myrothecium sp. Journal
of Antibiotics54, 517.
Wang Y, Hyde KD, McKenzie EHC, Jiang YL, Li
DW, Zhao DG (2015). Overview of
Stachybotrys (Memnoniella) and current
species status. Fungal Diversity71(1), 17–83.
Wang YU, Guo LD, Hyde KD (2005). Taxonomic
placement of sterile morphotypes of
endophytic fungi from Pinus tabulaeformis
(Pinaceae) in northeast China based on rDNA
sequences. Fungal Diversity 20: 235- 260.
Warpcup JH (1960). Methods for isolation and
estimation of activity of fungi in soil- In
Parkinson, D.P. andWaid, J.W. (eds.). The
ecology of soil fungi; p3-21. Liverpool
University press.
White t, Bruns T, Lee S and Taylor J (1990).
Amplification and direct sequencing of fungal
ribosomal RNA genes for phylogenetics. Inn;
Innis,M.,Gelfond,d.,Shinsky,I and
White,T.(eds.): PCR protocols: A guide to
methods and applications;p.315-322.Academic
Press,New York.
Zhao YJ, Li BJ, ShiYX and Xie XW (2009).
Identification of the pathogen of tomato
Myrotheciumleaf spot(in Chinese).Acta
Phytopathological ICA Sinica 39(5), 528–531.
Published
2020-12-15
How to Cite
ASSAF, L. H. (2020). MOLECULAR IDENTIFICATION AND INSECTICIDAL ACTIVITY OF Albifimbria verrucaria ISOLATED FROM CUCURBIT PLANTS AND SOIL IN IRAQ. Journal of Duhok University, 23(2), 106-113. https://doi.org/10.26682/ajuod.2020.23.2.13
Section
Agriculture and Veterinary Science
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