ISOLATION AND ANTIBIOTIC SUSCEPTIBILITY TEST OF PSEUDOMONAS MEMBERS IN THE PUS / WOUNDS INFECTIONS BY UTILIZING VITEK2 SYSTEM IN DUHOK CITY
Keywords:
Pseudomonas members, wound infections, antibiotic susceptibility test
Abstract
The aim of this study was to determine the rate of Pseudomonas species involved in wound infections. Also, the susceptibility and resistotyping patterns of the isolates to commonly prescribed antibiotics were also studied. During a period of nine months between March and November, 2022, a total of 200 specimens of pus or wound swabs were collected from adults patients that went to the Vin and Emergency Hospital in Duhok City. The samples were plated on Blood agar and MacConkey Agar, and the isolates were identified by the VITEK2 System. Antibiotic susceptibility and resistance profiles for 20 commonly prescribed antibiotics were also performed by the VITEK2 System. Out of the 200 samples collected from wound infections, 43 isolates of Pseudomonas species were identified at a rate of 21.5 % Pseudomonas aeruginosa being more common than the other species, which included 33 isolates (77 %), flowed by 7 P. putida isolates (16 %), and 3 P. fluorescens isolates (7 %). All isolates were obtained from adult patients aged 16–75 years, 29 (77%) of them were isolated from males, while 14 (33%) were isolated from females. The sensitivity patterns showed that Amikacin and Piperacillin-Tazobactam were the most active antibiotics against Pseudomonas aeruginosa, followed by Piperacillin, and then Colistin with Tobramycin. The Meropenem and imipenem show a high level of activity against P. putida and P. fluorescens isolates, while their sensitivity was moderate for Pseudomonas aeruginosa. Also, moderate sensitivity was revealed by Ciprofloxacin, Levofloxacin, and Ceftazidime, while all isolates were resistant to the Cefixime, Ceftriaxone, Cefazolin, Co-Trimethoprim, Amoxiclav, and Amoxiclav, and low sensitivity to Cefepime
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References
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J Pure Appl Microbiol., 16(1):430-434. doi: 10.22207/JPAM.16.1.39
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Gupta, E., Mohanty, S., Sood, S., Dhawan, B., Das, B. K., & Kapil, A. (2006). Emerging resistance to carbapenems in a tertiary care hospital in north India. The Indian journal of medical research, 124(1), 95–98.
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Ito, C. A. S., Bail, L., Arend, L. N. V. S., Nogueira, K. D. S., & Tuon, F. F. (2021). The activity of ceftazidime/avibactam against carbapenem-resistant Pseudomonas aeruginosa. Infectious diseases (London, England), 53(5), 386–389. https://doi.org/10.1080/23744235.2020.1867763
Javiya, V. A., Ghatak, S. B., Patel, K. R., & Patel, J. A. (2008). Antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India. Indian journal of pharmacology, 40(5),230–234. https://doi.org/10.4103/0253-7613.44156
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Kalluf, K. O., Arend, L. N., Wuicik, T. E., Pilonetto, M., & Tuon, F. F. (2017). Molecular epidemiology of SPM-1-producing Pseudomonas aeruginosa by rep-PCR in hospitals in Parana, Brazil. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 49, 130–133. https://doi.org/10.1016/j.meegid.2016.11.025
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Le, L., Baer, M., Briggs, P., Bullock, N., Cole, W., DiMarco, D., Hamil, R., Harrell, K., Kasper, M., Li, W., Patel, K., Sabo, M., Thibodeaux, K., & Serena, T. E. (2021). Diagnostic Accuracy of Point-of-Care Fluorescence Imaging for the Detection of Bacterial Burden in Wounds: Results from the 350-Patient Fluorescence Imaging Assessment and Guidance Trial. Advances in wound care, 10(3),123–136. https://doi.org/10.1089/wound.2020.1272
Lee, K., Lim, Y. S., Yong, D., Yum, J. H., & Chong, Y. (2003). Evaluation of the Hodge test and the imipenem-EDTA double-disk synergy test for differentiating metallo-beta-lactamase-producing isolates of Pseudomonas spp. and Acinetobacter spp. Journal of clinical microbiology, 41(10),4623–4629. https://doi.org/10.1128/JCM.41.10.4623-4629.2003
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Lister, P. D., Wolter, D. J., & Hanson, N. D. (2009). Antibacterial-resistant Pseudomonas aeruginosa: clinical impact and complex regulation of chromosomally encoded resistance mechanisms. Clinical microbiology reviews, 22(4),582–610. https://doi.org/10.1128/CMR.00040-09
Mutluoglu, M., & Uzun, G. (2011). Pseudomonas infection in a postoperative foot wound. CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne, 183(8), E499. https://doi.org/10.1503/cmaj.100556
Navaneeth, B. V., Sridaran, D., Sahay, D., & Belwadi, M. R. (2002). A preliminary study on metallo-beta-lactamase producing Pseudomonas aeruginosa in hospitalized patients. The Indian journal of medical research, 116, 264–267.
Obritsch, M. D., Fish, D. N., MacLaren, R., & Jung, R. (2004). National surveillance of antimicrobial resistance in Pseudomonas aeruginosa isolates obtained from intensive care unit patients from 1993 to 2002. Antimicrobial agents and chemotherapy, 48(12),4606–4610. https://doi.org/10.1128/AAC.48.12.4606-4610.2004
. Paterson, D. L., & Harris, P. N. (2016). Colistin resistance: a major breach in our last line of defence. The Lancet. Infectious diseases, 16(2), 132–133. https://doi.org/10.1016/S1473-3099(15)00463-6
Patzer, J. A., & Dzierzanowska, D. (2007). Increase of imipenem resistance among Pseudomonas aeruginosa isolates from a Polish paediatric hospital (1993-2002). International journal of antimicrobial agents, 29(2), 153–158. https://doi.org/10.1016/j.ijantimicag.2006.08.044
Perry, C. M., & Markham, A. (1999). Piperacillin/tazobactam: an updated review of its use in the treatment of bacterial infections. Drugs, 57(5), 805–843. https://doi.org/10.2165/00003495-199957050-00017
Poole K. (2005). Aminoglycoside resistance in Pseudomonas aeruginosa. Antimicrobial agents and chemotherapy, 49(2), 479–487. https://doi.org/10.1128/AAC.49.2.479-487.2005
Puri, J., Revathi, G., Kundra, P., & Talwar, V. (1996). Activity of third generation cephalosporins against Pseudomonas aeruginosa in high risk hospital units. Indian journal of medical sciences, 50(7), 239–243.
Rahim, K., Saleha, S., Zhu, X., Huo, L., Basit, A., & Franco, O. L. (2017). Bacterial Contribution in Chronicity of Wounds. Microbial ecology, 73(3),710–721. https://doi.org/10.1007/s00248-016-0867-9
Ranjan, K. P., Ranjan, N., Bansal, S. K., & Arora, D. R. (2010). Prevalence of Pseudomonas aeruginosa in post-operative wound infection in a referral hospital in Haryana, India. Journal of laboratory physicians, 2(2), 74–77. https://doi.org/10.4103/0974-2727.72153
Sangeeta Fattesingh Bhalavi, Vaishali Rahangdale, S.G. Joshi.( 2017), Study of antibiotic resistance pattern to urinary pathogens in a tertiary care hospital in central India. Int J Sci Res.; 6(8): 428-429.
Shah, D. A., Wasim, S., & Essa Abdullah, F. (2015). Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from urine samples of Urinary Tract Infections patients in Karachi, Pakistan. Pakistan journal of medical sciences, 31(2),341–345. https://doi.org/10.12669/pjms.312.6839
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Published
2023-05-17
How to Cite
ABDULLAH, B. H. (2023). ISOLATION AND ANTIBIOTIC SUSCEPTIBILITY TEST OF PSEUDOMONAS MEMBERS IN THE PUS / WOUNDS INFECTIONS BY UTILIZING VITEK2 SYSTEM IN DUHOK CITY. Journal of Duhok University, 26(1), 244-255. https://doi.org/10.26682/sjuod.2023.26.1.24
Section
Pure and Engineering Sciences
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