ANTIMICROBIAL ACTIVITY OF CHINABERRY Melia Azedarach EXTRACT AGAINST Pseudomonas syringae PV. syringae IN VITRO

  • WAZEER A. HASSAN Dept . of Plant Protection, College of Agriculture, University of Duhok, Kurdistan Region-Iraq
  • JIVAN M. M. AL-DOSKI Dept . of Plant Protection, College of Agriculture, University of Duhok, Kurdistan Region-Iraq
  • NEROJ Y. M. EBO Dept. of Forestry, College of Agriculture, University of Duhok, Kurdistan Region-Iraq
Keywords: Chainberry;, M. Azedarach;

Abstract

Using hot aqueous, cold aqueous and ethanol, the antimicrobial activity of both fresh leaves and chinaberry fruits extract were evaluated at 50, 100, 150, and 200 mg ml-1 against Pseudomonas syringae pv. syringae (Pss) growth on nutrient agar medium. Fruit extract was inhibited bacterial growth evidently with 8.84 mm in diameter, the hot aqueous and ethanol extracts showed a high antimicrobial potential when the inhibition zones reached to 8.68mm and 8.85mm, respectively. The results exhibited the imperceptible augmentation of inhibition zones of Pss growth with increasing extract doses (50, 100, 150, and 200) mg ml-1 and attained (6.72, 7.46, 8.89, and 10.37) mm, respectively. The highest and considerable inhibition zone 9.32 mm appeared using ethanol extract of fruit, the latter was more obvious on the bacterial development and inhibited its growth to 11.2mm when used at 200 mg ml-1, followed by leaf extract at the same concentration. Extracts at 200 mg ml-1 revealed substantial inhibition zone ranged between 10.37mm and 10.95mm using different methods. Therefore, the combination of ethanol extract of fruit at 200 mg ml-1 gave the maximum inhibition 11.7mm in diameter, followed by 10.97mm and 10.93mm when used hot and cold aqueous extracts.

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Published
2019-01-01
How to Cite
HASSAN, W. A., AL-DOSKI, J. M. M., & EBO, N. Y. M. (2019). ANTIMICROBIAL ACTIVITY OF CHINABERRY Melia Azedarach EXTRACT AGAINST Pseudomonas syringae PV. syringae IN VITRO. Journal of Duhok University, 21(1), 29-36. https://doi.org/10.26682/avuod.2019.21.1.4
Section
Agriculture and Veterinary Science