SEROLOGICAL AND BIOLOGICAL DETECTION OF TOMATO MOSAIC VIRUS IN SOIL AND SEEDS OF TOMATO PLANTS IN DUHOK PROVINCE

  • NABEEL AZIZ KASSIM Dept. of plant protection, Faculty of Agriculture and Forestry, University of Mosul -Iraq
  • WAZEER ALI HASSAN Dept. of plant protection, Collage of Agriculture Engineering Sciences, University of Duhok, Kurdistan region-Iraq
  • VIYAN JAMEEL HACI Dept. of plant protection, Collage of Agriculture Engineering Sciences, University of Duhok, Kurdistan region-Iraq
Keywords: Tomato:, Tomato mosaic virus:, DAS-ELISA:

Abstract

Tomato mosaic virus (ToMV) is one of the most important viruses on tomato; contaminated soil may be the main source and reservoir of transmission. Diseased plants were collected from Kamona and Xanike villages, Duhok province. DAS-ELISA technique its detection in the soil and contaminated seeds, and clarify the virus transmission from contaminated seeds to seedlings. Using indicator plants (Biological detection) was to identify virus from contaminated soil. The result showed that six samples out of 14 soil samples gave a positive reaction with 42.8% is transmission ratio from soil. Symptoms on indicator plants such as:  mosaic, malformation, vein clearing on C. qinoa; the stunted plants with localized necrotic lesions and malformation on pepper plants. DAS-ELISA from tomato seeds displayed in 11 samples out of 18 samples revealed strong reactions and seeds transmission proportion were 61%. Seedlings were germinated from 60 infected tomato seeds under plastic house condition but only 4 transplants exhibited ToMV symptoms of mosaic, and the percentage of transmission was 7.8%.

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References

Agdia-EMEA (2018). Easy-to-use test kits, reagents and laboratory testing services for agricultural diagnostics. www.agdia-emea.com.

Arinaitwe, W. (2013). Incidence, symptom severity and distribution of tomato viral diseases in Uganda. M.Sc. Thesis, Makerere University. Kampala, Uganda.70pp.

Almeida J. E.M., Figureira A. D.R., Duarte P.S.G., Lucas M.A. and Alencar N.E. (2018). Procedure for detecting Tobamovirus in tomato and pepper seeds decreases the cost analysis. Bragantia, vol.77, No. 4, p. 590-598.

Broadbent, L. (1965). The epidemiology of tomato mosaic XI. Seed transmission of TMV. Ann. Appl. Biol. 56:177-205.

Broadbent, L., 1976. Epidemiology and control of Tomato mosaic virus. Annual Reviews of Phytopathology, 14:76-96.

Broadbent, L., W. H. Read, and F. T. Last. 1965. The epidemiology of tomato mosaic. X. Persistence of TMV-infected debris in soil, and the effects of soil partial sterilization. Ann. Appl. Biol. 55:471–483.

Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. & Zurcher, E.J. (eds.). (1997). `Plant Viruses Online: Descriptions and Lists from the VIDE Database.http://sdb.im.ac.cn/vide/descr832.htm.

Buttner, C. and Nienhaus, F. (1989). Virus contamination of soils in forest ecosystems of the Federal Republic of Germany. - Eur. J. Forest Pathol. 19: 47-53.

Chitra T.R., Prakash H.S., Albrechtsen S.E., Shetty H.S., Mathur S.B., 1998. Seed transmission of ToMV in tomato and TMV in bell pepper. Tropical Science (in press).

Chitra, T.R., H.S. Prakash, S.E. Albrechtsen, H.S. Shetty, and S.B. Mathur. 1999. Infection of tomato and bell pepper by ToMV and TMV at different growth stages and establishment of virus in seeds. J. Plant Pathol. 81:123-126.

Chookhampaeng, S., Pattanagul W. and Theerakulpisut, P., 2008. Effects of Salinity on Growth, Activity of Antioxidant Enzymes and Sucrose Content in Tomato (Lycopersicon esculentum Mill.) at the Reproductive Stage. Science Asia 34: 069-075.

Clark, M. F. and A. N. Adams (1977) Characterization of the microplate method of the enzyme-linked immunosorbent assay for the detection of plant virus. J. Gen. Virol., 34:475-483.

de Assis Filho, F.M. and Sherwood, J.L. 2000. Evaluation of seed transmission of turnip yellow mosaic virus and tobacco mosaic virus in Arabidopsis thaliana. Phytopatho. 90:1233-1238.

Dorais M, Papadopoulos AP, Gosselin A, 2001. Influence of electric conductivity management in greenhouse tomato yield and fruit quality. Agronomie 21:367-383.

Dorais, M., Ehret, D.L., Papadopoulos, A.P. (2008): Tomato (Solanum lycopersicum) health components: from the seed to the consumer. - Phytochem Rev. 7: 231-250.

Fillhart R.C., Bachand G.D. and Castello J.D., (1998). Detection of Infectious Tobamoviruses in Forest Soils. Applied and environmental microbiology, vol.64, No.4, p. 1430–1435.

Francki, R.I.B., R.G. Milne and T. Hatta. 1985. Atlas of Plant Virus. Vo. II, CRC Press, Florida, 284-285.

Gooding Jr, G.V. and E.G. Suggs. 1976. Seedborne tobacco mosaic virus in commercial sources of tomato seed. Plant Dis. Rept. 60:441-442.

Hadas, R., Pearlsman, M., Gefen, T., Lachman, O., Hadar, E. and Sharabany, G., (2004). Note: Indexing system for Tomato Mosaic Virus (ToMV) in commercial tomato seed lots. Phytoparasitica, vol. 32, No.4, p.421-424.

Hall, T.J. 1980. Resistance at Tm-2 locus in tomato to Tomato mosaic virus. Euphytica 29:189-197.
Hameed, S. 1995. Leaf curl virus resistance in tomato and chilies. Final Report, South Asian Vegetable Research Network. Virology Section (CDRI), NARC, Islamabad.
Hanssen IM, Lapidot M, Thomma BPHJ (2010) Emerging viral diseases of tomato crops. Mol Plant Microbe Interact 23:539-548.

Hoggan, I. A.. 1. Johnson (1936): Behaviour of the ordinary Tobacco mosaic virus in the soil. J. Agric. Res. 52, 271 294.

Hollings, M., and H. Huttinga. 1976. Tomato mosaic virus. Descriptions of plant viruses, no. 156. Commonwealth Mycological Institute/Association for Applied Biology, Kew, England.

Kegler, H., Fuchs, E., Spaar, D. and Kegler, J. (1995) Viren in Boden und Grundwasser (Viruses in soil and groundwater). Archives of Phytopathology and Plant Protection 29, 349–371.

Khan, I.A. 1997. Occurrence, distribution, host range, symptomoly and purification of ToMV on tomato. Pak. J. Zool. 29(4): 385-389.
King, A.M., M.J., Adams, E.J. Lefkowitz and E.B. Carstens. 2011. Virus taxonomy: IXth report of the International Committee on Taxonomy of Viruses.

Kumar, S., Udaya Shankar, A. C., Nayaka, S. C., Lund, O. S. and Prakash, H. S. (2011). Detection of Tobacco mosaic virus and Tomato mosaic virus in pepper and tomato by multiplex RT-PCR. Letters in Applied Microbiology. Vol.53, No.3, p. 359–363.

Lanter, J. M., J. M. McGuire, and M. J. Goode. 1983. Persistence of Tomato mosaic virus in tomato debris and soil under field conditions. Plant Dis. 66:552–555.

Li R, Gao S, Fei Z, Ling KS (2013). Complete genome sequence of a new tobamovirus naturally infecting tomatoes in Mexico. Genome Announc 1(5). doi:10.1128/genomeA.00794-13.

Madhusudhan K.N., Nalini M.S., Prakash H.S. and Shetty H.S. (2005). Effect of Inducer against Tobamovirus Infection in Tomato and Bell Pepper. International Journal of botany 1(1), p. 59-61.

Morrison R.H. 1999. Sampling in seed health testing // phytopathology., vol.89, p. 1084-1087.

Nagai Y., (1981). Control of mosaic disease of tomato and sweet pepper caused by tobacco mosaic virus. Spec.Bull. Chiba Agric. Exp. Stn. 9: 1-109 (in Japanese with: English summary).

Pares, R.D. and Gunn, L.V. (1989). The role of non-vectored soil transmission as a primary source of infection by pepper mild mottle and cucumber mosaic viruses in greenhouse-grown capsicum in Australia. J. Phytopathol. 126: 353-360.

Pelham, J. 1966. Resistance in tomato to tobacco mosaic virus. Euphytica 15:258-267.

Petrov N. (2014). Damaging effects of tomato mosaic virus and potato virus Y on tomato plant. Science & Technologies.Vol. IV, No. 6.

Pradhanang P. M. , Greonwegen K. and Ozminkowski et al., 2005. Tobacco mosaic virus: does it really transmit through tomato seed? (Abstract): 5th ISTA-SCH seed health symposium.- Angers, France.

Sevİk, M. A and Kose-tohumcu, E. (2011). The ELISA analysis results in tomato (Lycopersicon esculentum Mill) seed health testing for Tobacco mosaic virus. Zemdirbyste Agriculture. vol. 98, No. 3, p. 301–306.

Sutic, D.D., R.E. Ford and M.T. Tosic. 1999. Handbook of Plant Virus Diseases. CRC Press.

Najeeb Ullah, N., Ali, A., Ahmad, M., Fahim, M., Dini,N. and Ahmad, F. (2017). Evaluation of tomato genotypes against tomato mosaic virus (ToMV) and its effects on yield contributing parameters. Pak. J. Bot., 49(4): 1585-1592.

Van Winckel, A. and Gcypens, M. (1965). Inactivering van Tabakmosaiekvirus in de grond. Parasitica, 11, 124-137.

Yadav K. And Yadav P., (2017). The effect of different mechanism of transmission on transavaibility of Tomato Mosaic Virus. Journal of Pharmacognosy and Phytochemistry,Vol. 6,No. 6, p. 448-450.

Yang, J.G, Wang, F.L., Chen D.X., Shen, L.L., Qian Y.M., Liang Z.Y., Zhou W.C. and Yan T.H. (2012). Development of a one-step immunocapture real-time RT-PCR assay for detection of Tobacco mosaic virus in soil. Sensors (Switzerland), vol. 12, No.12, p. 16685-16694.
Published
2020-02-13
How to Cite
NABEEL AZIZ KASSIM, WAZEER ALI HASSAN, & VIYAN JAMEEL HACI. (2020). SEROLOGICAL AND BIOLOGICAL DETECTION OF TOMATO MOSAIC VIRUS IN SOIL AND SEEDS OF TOMATO PLANTS IN DUHOK PROVINCE. Journal of Duhok University, 22(2), 1-8. https://doi.org/10.26682/ajuod.2019.22.2.1
Section
Agriculture and Veterinary Science