DETECTION OF GRAPEVINE (Vitis Vinifera) RESISTANT VARIETIES TO POWDERY MILDEW DISEASE BASED ON SSR-PCR MARKER

ROJAN SH.  SULAIMAN; AVESTA MOHAMMED  ALI; PAYMAN. A. A.  ZIBARI

doi:10.26682/ajuod.2020.23.1.4

ROJAN SH. SULAIMAN Dept. of Horticultural, Collage of Agriculture Engineering Sciences, University of Duhok
AVESTA MOHAMMED ALI Dept. of Horticultural, Collage of Agriculture Engineering Sciences, University of Duhok
PAYMAN. A. A. ZIBARI Scientific Research Center, College of Science, University of Duhok

DOI: https://doi.org/10.26682/ajuod.2020.23.1.4

Keywords: Grape vine, Powdery mildew, SSR Marker, Vitis vinifera

Abstract

Powdery mildew is a fungal disease caused by Erysiphe necator considered to be most important fungal diseases and causing loss of grapevine production. The use of molecular markers has been proposed to be the best method for identifying genes for resistance to powdery mildew which provides basic information in breeding programs. This study has been conducted at Scientific Research Center/College of Science/University of Duhok during the period (July 2018 to August 2019). Simple Sequence Repeat (SSR) Marker has been used to detect the powdery mildew by applying eleven SSR markers for twenty cultivars of Vitis vinifera
collected from Duhok vineyard and Directorate of Horticulture in Duhok City. Considered the useful markers as the guidelines for detecting the gene responsible for the resistance to powdery mildew, in this study all the varieties and land races that had been used to detect such a gene were

Downloads

Download data is not yet available.

References

Abdul-Qader, S. M., & Alsaidi, I. H. (2006). Effect of Training System, Canopy Management and Dates on the Yield and Quality of Grapevines cv.‗ Taifi ‘(Vitis vinifera L.) Under non Irrigated Condition. M. Sc. Thesis, College of Agric. Univ. of Duhok, Iraq. pp: 40-55.
Aigrain, P. (1999). Conjoncture vitivinicole mondiale. Bull. OIV, 817(81), 228-240.
Al-Atrushi, S. M. (2009). Effect of eyes number and foliar spray of Potassium and Copper on the vegetative growth, productivity and quality of Grape (Vitis vinifera L.) cv. Zark under non-irrigated condition. Ph. D. Thesis. The Council of the College of Agriculture and Forestry ….
Alsaidi, I. (2000). Grape production: Mosul university press.
Carputo, D. (2015). Discovery and genotyping of DNA sequence variations in grape.
Ficke, A., Gadoury, D. M., & Seem, R. C. (2002). Ontogenic resistance and plant disease management: A case study of grape powdery mildew. Phytopathology, 92(6), 671-675.
Fundyga, R. E., Lott, T. J., & Arnold, J. (2002). Population structure of Candida albicans, a member of the human flora, as determined by microsatellite loci. Infection, Genetics and Evolution, 2(1), 57-68.
Gadoury, D. M., Seem, R. C., Ficke, A., & Wilcox, W. F. (2003). Ontogenic resistance to powdery mildew in grape berries. Phytopathology, 93(5), 547-555.
Gadoury, D. M., Seem, R. C., Pearson, R. C., Wilcox, W. F., & Dunst, R. M. (2001). Effects of powdery mildew on vine growth, yield, and quality of concord grapes. Plant disease, 85(2), 137-140.
Goto-Yamamoto, N., Mouri, H., Azumi, M., & Edwards, K. J. (2006). Development of grape microsatellite markers and microsatellite analysis including oriental cultivars. American journal of enology and viticulture, 57(1), 105-108.
Hoffmann, S., Di Gaspero, G., Kovács, L., Howard, S., Kiss, E., Galbács, Z., Kozma, P. (2008). Resistance to Erysiphe necator in the grapevine ‘Kishmish vatkana’is controlled by a single locus through restriction of hyphal growth. Theoretical and Applied Genetics, 116(3), 427-438.
Laucou, V., Lacombe, T., Dechesne, F., Siret, R., Bruno, J.-P., Dessup, M., Roux, C. (2011). High throughput analysis of grape genetic diversity as a tool for germplasm collection management. Theoretical and Applied Genetics, 122(6), 1233-1245.
Montarry, J., Cartolaro, P., Delmotte, F., Jolivet, J., & Willocquet, L. (2008). Genetic structure and aggressiveness of Erysiphe necator populations during grapevine powdery mildew epidemics. Appl. Environ. Microbiol., 74(20), 6327-6332.
Panstruga, R., & Schulze‐Lefert, P. (2002). Live and let live: insights into powdery mildew disease and resistance. Molecular plant pathology, 3(6), 495-502.
Riaz, S., Tenscher, A., Ramming, D., & Walker, M. (2011). Using a limited mapping strategy to identify major QTLs for resistance to grapevine powdery mildew (Erysiphe necator) and their use in marker-assisted breeding. Theoretical and Applied Genetics, 122(6), 1059-1073.
Scott, E., Dambergs, R., & Stummer, B. (2010). Fungal contaminants in the vineyard and wine quality Managing wine quality (pp. 481-514): Elsevier.
Sefc, K., Lopes, M., Lefort, F., Botta, R., Roubelakis-Angelakis, K., Ibanez, J., . . . Steinkellner, H. (2000). Microsatellite variability in grapevine cultivars from different European regions and evaluation of assignment testing to assess the geographic origin of cultivars. Theoretical and Applied Genetics, 100(3-4), 498-505.
Sefc, K. M., Steinkellner, H., Wagner, H., Glössl, J., & Regner, F. (1997). Application of microsatellite markers to parentage studies in grapevine. Vitis, 36(4), 179-183.
This, P., Jung, A., Boccacci, P., Borrego, J., Botta, R., Costantini, L., Ferreira-Monteiro, F. (2004). Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theoretical and Applied Genetics, 109(7), 1448-1458.
Veikondis, R. (2014). Genetic characterisation of fungal disease resistance genes in grapevine using molecular marker technology. Stellenbosch: Stellenbosch University.
Weigand, F., Baum, M., & Udupa, S. (1993). DNA molecular marker techniques. Technical Manual No. 20: ICARDA.