THE RESPONSE OF IN VITRO PROPAGATION OF MARUMI KUMQUAT (FORTUNELLA JAPONICA THUNB.) TO DIFFERENT CULTURE MEDIA, PLANT GROWTH REGULATORS AND DIFFERENT FRUCTOSE CONCENTRATION

  • JEILLAN H. HUSSEIN Dept. of Biology, College of Sciences, University of Duhok, Kurdistan Region-Iraq
  • DIAA A. IBRAHIM Scientific Research Center, College of Science, University of Duhok, Kurdistan Region-Iraq
Keywords: Fortunella japonica; Micropropagation; Shoot multiplication; Fructose; Explant; Plant Growth Regulator

Abstract

Marumi kumquat (Fortunella Japonica) is culture for its valuable nutritional value and medicinal importance in many regions of the world. The current study aimed to evaluate the effect of two types of media enriched with different concentrations of fructose and different plant growth regulators and different fructose concentration on in vitro propagation of Fortunella Japonica. The findings showed that the most effective treatment for explant surface sterilization was by using 0.1% HgCl2 for ten minutes which give best results for production contamination-free explants at the initiation cultures. At multiplication stage, WPM medium gave better results at all tested BA levels as compared with MS medium. No significant differences were showed by using BA alone or in combination with GA3 in the measured parameters. It has been observed that WPM medium supplemented with 0.5mgl-1 BA with the presence of 30mgl-1 fructose was able to give the highest shoot length (1.56cm) with maximum shoots number/explant 9.0 and highest leaves number/explant (21.0). The proliferated shoots were exposed to full strength MS medium salts supplemented with 2mgl-1 NAA which showed the highest ratio of rooting. In vitro rooted plantlets were gradually acclimatized and transferred to open air conditions, which recorded a high survive rate reached to 92%.

 

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Published
2020-09-14
How to Cite
HUSSEIN, J. H., & IBRAHIM, D. A. (2020). THE RESPONSE OF IN VITRO PROPAGATION OF MARUMI KUMQUAT (FORTUNELLA JAPONICA THUNB.) TO DIFFERENT CULTURE MEDIA, PLANT GROWTH REGULATORS AND DIFFERENT FRUCTOSE CONCENTRATION . Journal of Duhok University, 23(1), 178-190. https://doi.org/10.26682/sjuod.2020.23.1.19
Section
Pure and Engineering Sciences