STUDY GENETIC RELATIONSHIP AMONG ENTEROCOCCUS FAECALIS STRAINS COLLECTED FROM URINE HARBORED DIFFERENT VIRULENCE PROFILES USING ERIC-PCR ASSAY

  • WASAN M. ALNAKSHABANDI Anesthesia Science, College of Health Science, University of Duhok, Kurdistan Region-Iraq
  • NARMIN SAEED MERZA Dept. of Biology,College of Science,University of Duhok, Kurdistan Region-Iraq
  • HAVAL MOHAMMED KHALED Dept. of Biology,College of Science,University of Zakho, Kurdistan Region-Iraq
  • JALADET M. S. JUBRAEL cientific Research Center, College of Science,University of Duhok, Kurdistan Region-Iraq
Keywords: KEYWORDS: ERIC, Enterococcus,  faecalis,  PCR, Virulence Genes

Abstract

ABSTRACT

    Twenty five isolates of Enterococcus faecalis have been previously identified and characterized were subjected to ERIC-PCR analysis in order to study the genetic relationship with regarding to their virulence profile. Nine virulence profiles were identified according to the presence/ absence of five virulence factors; asa1,gelE, esp, cpd and ace. Most isolates (28%) were belonged to Virprofile1 asa1+,gelE+, esp+, cpd+,ace+ followed by Virprofile3asa1+,gelE-, esp+, cpd+,ace+ (20%), Virprofile4 asa1+,gelE+, esp-, cpd+,ace- accounting 16%, while virprofiles5 asa1+,gelE-,esp-,cpd+,ace+, virprofile6 asa1-,gelE+,esp+,cpd+,ace+ and virprofile8 asa1+,gelE-, esp+, cpd+,ace- representing 8%, virprofile2 asa1-,gelE-,esp-, cpd-, ace+, virprofile7 asa1+,gelE+, esp+, cpd+,ace-  and virprofile9 asa1+,gelE+, esp-, cpd+,ace+ were  4%. ERIC-PCR analysis divided isolates into two main clusters named; cluster A accounting 28% which further classified into groups; 8% isolates were belonged to A1 and 20% were belonged to A2. Most isolates were belonged to cluster B accounting 72%. This cluster was involved two groups; six isolates (33.3%) were belonged to B1 while 66.6% of isolates were assigned as B2. However, no relationship was found between virulence profiles with phylogentic groups  of the isolates.

 

 

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References

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Published
2020-06-07
How to Cite
ALNAKSHABANDI, W. M., MERZA, N. S., KHALED, H. M., & S. JUBRAEL, J. M. (2020). STUDY GENETIC RELATIONSHIP AMONG ENTEROCOCCUS FAECALIS STRAINS COLLECTED FROM URINE HARBORED DIFFERENT VIRULENCE PROFILES USING ERIC-PCR ASSAY. Journal of Duhok University, 23(1), 63-68. https://doi.org/10.26682/sjuod.2020.23.1.7
Section
Pure and Engineering Sciences