• Journal of Duhok University UoD
  • ZULAYKHA ABDULWAHAB ABDULJALIL Dept. of Plant Protection, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region-Iraq
  • NABEEL AZIZ KASSIM Dept. of Plant Protection, College of Agriculture and Forestry, University of Mosul-Iraq
  • MOSLEH MOHAMMAD SAEED DUHOKY Dept. of Plant Protection, College of Agricultural Engineering Sciences, University of Duhok, Kurdistan Region-Iraq
Keywords: Dahlia pinnata, Dahlia mosaic virus, PCR, Biological studies


The presence of Dahlia mosaic virus (DMV) was confirmed for the first time on both leaves and tubers of Dahlia pinnata in Iraq. DMV was confirmed by amplification of opening reading frame 1 (ORF1) that is encoding for the movement protein, using the specific primer (DMV F1 and DMV R1). The expected size of about 900 bps was amplified from 30 samples. There was no evidence of infection with DMV-D10 and dahlia common mosaic virus. Mechanical and vector transmission studied as biological properties of the virus. D. pinnata, Zinnia elegans, Ageratum conyzoides cv. Blue Ball, Amaranthus caudates, Helianthus annuus and Chrysanthemum indicum were used as indicator plants. DMV was mechanically transmissible to all tested plants, at a rate of about 80%. The diseased dahlia was tested for the presence of viral infections with, TSV, TRV, CMV and TSWV using Chenopodium quinoa and Nicotiana tabacum as diagnostic species. Both species remained symptomless after several inoculations. DMV was transmitted by Myzus persicae and Aphis fabae at the rate of 60 and 55 %, respectively


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Author Biography

Journal of Duhok University UoD

Journal of Duhok University

University of Duhok

Kurdistan Region - Iraq


Abdel-Salam, A.M.; Al Khazindar M.M.; Eid S.G., and Pappu H.R. 2010. Caulimoviral sequences in Dahlia variabilis in Egypt. African Journal of Biotechnology 9(41): 6835-6839.

Abtahi, F.S. and Koohi Habibi M. 2008. Host range and some characterization of Tobacco streak virus isolated from lettuce in Iran. African Journal of Biotechnology 7(23):4260-4264.

Ayo-John, E.I. and Hughes J.d`A. 2014. Identification of Cucumber mosaic virus (CMV) Isolates Infecting Musa spp. and Vegetable Crops in Southern Nigeria. International Journal of Virology 10: 204-210.

Blanc, S. 2008. Vector transmission of plant viruses. Encyclopedia of Virology (eds B.W.J. Mahy and M.H.V. van regenmortel), pp. 274–282. Academic Press, New York.

Brunt, A.A. 1971. Description of plant viruses. Dahlia Mosaic Virus. Annual applied of Biology 67: 357.

Brunt, A.A.; Crabtree, K.; Dallwitz, M.J.; Gibbs, A.J.; Watson, L., and Zurcher, E.J. 1996. `Plant viruses online: Descriptions and lists from the VIDE database.

Eid S. and Pappu H.R. 2014. Biological studies of three caulimoviruses associated with dahlia (Dahlia variabilis). Canadian Journal of Plant Pathology 36(1):110-115.

French, J.M., Goldberg, N.P., Randall, J.J. and Hanson, S.F. 2015. New Mexico and the southwestern US are affected by a unique population of tomato spotted wilt virus (TSWV) strains. Archives of Virology. DOI: 10.1007/s00705-015-2707-5

Hamed A.H., El-Banna Om-Hashim M., Ghanem G.A.M., Elnagaar H. and Shafie M.S. 2012. Isolation and identification of Tobacco rattle tobravirus affecting onion (Allium cepa L.) plants in Egypt. International Journal of Virology, 8: 39-49.

Lopez, M.M.; Llop, P.; Olmos, A.; Marco-Noales, E.; Cambra, M., and Bertolini, E. 2008. Are molecular tools solving the challenges posed by detection of plant pathogenic bacteria and viruses? Current Issues in Molecular Biology, 11:13-45.

Nerway, Zulaykha, A.A., Toma Rafail, S. and Kassim Nabeel A. 2012. Identification and Purification of Potato virus Y (PVY) in Duhok Governorate. Journal of University of Duhok, 15(1): 413-422.

Makkouk, K.M., and Kumari, S.G. 2006. Molecular diagnosis of plant viruses. Arab Journal of Plant Protection, 24: 135-138.

McCartney, A.H.; Foster, S.J.; Fraaige, B.A., and Ward, E. 2003. Mo¬lecular diagnostics for fungal plant pathogens. Pest Management Science, 59:129-142.

Pahalawatta V., Druffel K.L., Wyatt S.D., Eastwell K.C. and Pappu H.R. 2008a. Genome structure and organization of a member of a novel and distinct species of the genus Caulimovirus associated with Dahlia mosaic. Archive of Virology, 153, 733–738.

Pappu, H.R., Wyatt S.D., and Druffel K.L. 2005. DMV: Molecular detection and distribution in dahlia in the US. HortScience, 40:697-699.

Salem, N.M., Mansour A. and Badwan H. 2012. Identification and partial characterization of Tomato spotted wilt virus on lettuce in Jordan. Journal of Plant Pathology, 94(2), 431-435.
Schaad, N.W., and Frederick, R.D. 2002. Real-time PCR and its ap¬plication for rapid plant disease diagnostics. Canadian Journal of Plant Pathology, 24: 250-258.

Webster, C.G.; Wylie, J.S., and Jones, M.G.K. 2004. Diagnosis of plant viral pathogens. Current Science, 86: 1604-1607.

Whitley, G.R. 1985. The medicinal and nutritional properties of Dahlia spp. Journal of Ethnopharmacology 14(1):75-82.
How to Cite
UoD, J. of D. U., ABDULJALIL, Z. A., KASSIM, N. A., & DUHOKY, M. M. S. (2020). FIRST RECORD OF DAHLIA MOSAIC VIRUS IN IRAQ ON DAHLIA (Dahlia pinnata). Journal of Duhok University, 22(2), 113-120.
Agriculture and Veterinary Science

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